In vitro kinetic study of growth and mineralization of osteoblast-like cells (Saos-2) on titanium surface coated with a RGD functionalized bisphosphonate

  1. Johanne Beuvelot1,
  2. David Portet2,
  3. Grégory Lecollinet2,
  4. Marie-Françoise Moreau1,
  5. Michel Félix Baslé1,
  6. Daniel Chappard1,
  7. Hélène Libouban1,*

Article first published online: 7 APR 2009

DOI: 10.1002/jbm.b.31357

Issue

Journal of Biomedical Materials Research Part B: Applied Biomaterials

Journal of Biomedical Materials Research Part B: Applied Biomaterials

Volume 90B, Issue 2, pages 873–881, August 2009

Additional Information

How to Cite

Beuvelot, J., Portet, D., Lecollinet, G., Moreau, M.-F., Baslé, M. F., Chappard, D. and Libouban, H. (2009), In vitro kinetic study of growth and mineralization of osteoblast-like cells (Saos-2) on titanium surface coated with a RGD functionalized bisphosphonate. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 90B: 873–881. doi: 10.1002/jbm.b.31357

Author Information

  1. 1

    INSERM, U922, LHEA, Faculté de Médecine, Angers, Cedex 49045, France

  2. 2

    SURFACTIS Technologies, Angers, Cedex 49100, France

*INSERM, U922, LHEA, Faculté de Médecine, Angers, Cedex 49045, France

Publication History

  1. Issue published online: 1 JUL 2009
  2. Article first published online: 7 APR 2009
  3. Manuscript Accepted: 8 JAN 2009
  4. Manuscript Revised: 13 NOV 2008
  5. Manuscript Received: 18 JUL 2008

Funded by

  • Contrat de Plan Etat, Région “Pays de la Loire” (Bioregos), INSERM

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (314K)

Keywords:

  • titanium;
  • adhesion molecule;
  • bisphosphonate;
  • osteoblast;
  • surface treatment

Abstract

Osteoconduction and osseointegration are the critical stages for implantation success. Peptides containing RGD (Arg-Gly-Asp) adhesive sequence are known to promote cell adhesion and consequently to favor osseointegration of medical devices. In this study, RGD peptides were coupled to a bisphosphonate used as an anchor system and chemically adsorbed on polished titanium discs. Two different concentrations, 10−10 mol/L (RGD 10−10) and 10−4 mol/L (RGD 10−4) were compared to non coated discs (RGD 0). Adhesion, spreading, and mineralization of osteoblast-like cells (Saos-2) were assessed. Mineralization kinetic was done at 3, 6, 10, 14, and 18 days of culture; the extent of mineral deposits was quantified by image analysis. Histogram repartitions of nuclear area, characterizing cell spreading, showed a shift to higher values in cells cultured on RGD coated titanium disks. Mineralization started at day 3 in the three groups, but had a faster development in the RGD 10−10 group from day 6 to day 18 compared to RGD 0 and RGD 10−4. At day 18, the percentage of mineralized area was significantly higher for RGD 10−10 compared to RGD 0 (p < 0.05). In the present study, this new method was found suitable to anchor RGD containing species on titanium: this favored adhesion and spreading of osteoblast-like cells and mineralization compared to noncoated titanium. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2009

View Full Article (HTML) Get PDF (314K)