3D collagen scaffolds coated with multiwalled carbon nanotubes: Initial cell attachment to internal surface

Authors

  • Eri Hirata,

    Corresponding author
    1. Department of Oral Functional Prosthodontics, Division of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Kita 13, Nishi 7, Kita-ku, Sappro 060-8586
    • Department of Oral Functional Prosthodontics, Division of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Kita 13, Nishi 7, Kita-ku, Sappro 060-8586
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  • Motohiro Uo,

    1. Department of Biomedical, Dental Materials and Science, Graduate School of Dental Medicine, Hokkaido University, Kita 13, Nishi 7, Kita-ku, Sappro 060-8586
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  • Yoshinobu Nodasaka,

    1. Support Section for Education and Research, Graduate School of Dental Medicine, Hokkaido University, Kita 13, Nishi 7, Kita-ku, Sappro 060-8586
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  • Hiroko Takita,

    1. Support Section for Education and Research, Graduate School of Dental Medicine, Hokkaido University, Kita 13, Nishi 7, Kita-ku, Sappro 060-8586
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  • Natsumi Ushijima,

    1. Support Section for Education and Research, Graduate School of Dental Medicine, Hokkaido University, Kita 13, Nishi 7, Kita-ku, Sappro 060-8586
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  • Tsukasa Akasaka,

    1. Department of Biomedical, Dental Materials and Science, Graduate School of Dental Medicine, Hokkaido University, Kita 13, Nishi 7, Kita-ku, Sappro 060-8586
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  • Fumio Watari,

    1. Department of Biomedical, Dental Materials and Science, Graduate School of Dental Medicine, Hokkaido University, Kita 13, Nishi 7, Kita-ku, Sappro 060-8586
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  • Atsuro Yokoyama

    1. Department of Oral Functional Prosthodontics, Division of Oral Functional Science, Graduate School of Dental Medicine, Hokkaido University, Kita 13, Nishi 7, Kita-ku, Sappro 060-8586
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Abstract

The cell adhesion in a multiwalled carbon nanotube-coated collagen sponge (MWCNT-coated sponge) was investigated. Immediately after seeding, the cells adhered to the inner surface of the MWCNT-coated sponge and a significantly larger number of cells were observed there than for a pure collagen sponge used as control. On the MWCNT-coated sponge, the cells appeared favorable adhesion and spread in the early stages in the center part of the sponge which cells rarely attached without MWCNT-coating. It was suggested that the physical structure of MWCNTs was effective for initial adhesion of cells from the result of serum-free culture. MWCNT-coating makes the material a suitable three-dimensional scaffold for cell culturing, as opposed to other scaffold systems where such an effect is not seen. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010

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