Get access

Synthesis, characterization of calcium phosphates/polyurethane composites for weight-bearing implants

Authors

  • Toshitaka Yoshii,

    1. Department of Orthopaedics and Rehabilitation, Vanderbilt University Medical Center, Nashville, Tennessee 37232-8774
    2. Center for Bone Biology, Vanderbilt University Medical Center, 2215B Garland Avenue, Nashville, Tennessee 37232-0575
    3. Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee 37235
    4. Section of Orthopaedic and Spinal Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo 113-8519, Japan
    Search for more papers by this author
  • Jerald E. Dumas,

    1. Center for Bone Biology, Vanderbilt University Medical Center, 2215B Garland Avenue, Nashville, Tennessee 37232-0575
    2. Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee 37235
    Search for more papers by this author
  • Atsushi Okawa,

    1. Section of Orthopaedic and Spinal Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo 113-8519, Japan
    Search for more papers by this author
  • Dan M. Spengler,

    1. Department of Orthopaedics and Rehabilitation, Vanderbilt University Medical Center, Nashville, Tennessee 37232-8774
    Search for more papers by this author
  • Scott A. Guelcher

    Corresponding author
    1. Center for Bone Biology, Vanderbilt University Medical Center, 2215B Garland Avenue, Nashville, Tennessee 37232-0575
    2. Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee 37235
    • Department of Orthopaedics and Rehabilitation, Vanderbilt University Medical Center, Nashville, Tennessee 37232-8774
    Search for more papers by this author

  • How to cite this article: Yoshii T, Dumas JE, Okawa A, Spengler DM, Guelcher SA. 2012. Synthesis, characterization of calcium phosphates/polyurethane composites for weight-bearing implants. J Biomed Mater Res Part A 2012:100B:32-40.

Abstract

Calcium phosphate (CaP)/polymer composites have been studied as an alternative graft material for the treatment of bone defects. In this study, lysine-triisocyanate-based polyurethane (PUR) composites were synthesized from both hydroxyapatite (HA) and β-tricalcium phosphate (TCP) to reduce the brittleness of CaP and increase the bioactivity of the polymer. The mechanical properties and in vitro cellular response were investigated for both HA/PUR and TCP/PUR composites. The composites were implanted in femoral defects in rats, and in vivo bioactivity was evaluated by X-rays, micro-computed tomography (μCT), and histological sections. In biomechanical testing, PUR improved the mechanical properties of the CaP, thus rendering it potentially suitable for weight-bearing applications. In vitro cell culture studies showed that CaP/PUR composites are biocompatible, with β-TCP enhancing the cell viability and proliferation relative to HA. CaP/PUR composites also supported the differentiation of osteoblastic cells on the materials. When implanted in rat femoral defects, the CaP/PUR composites were biocompatible and osteoconductive with no adverse inflammatory response, as evidenced by X-rays, μCT images, and histological sections. Additionally, a histological examination showed evidence of cellular infiltration and appositional remodeling. These results suggest that CaP/PUR composites could be potentially useful biomaterials for weight-bearing orthopaedic implants. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.

Get access to the full text of this article

Ancillary