How to cite this article: Zhou M, Liu Z, Liu C, Jiang X, Wei Z, Qiao W, Ran F, Wang W, Qiao T, Liu C. 2012. Tissue engineering of small-diameter vascular grafts by endothelial progenitor cells seeding heparin-coated decellularized scaffolds. J Biomed Mater Res Part B 2012:100B:111-120.
Tissue engineering of small-diameter vascular grafts by endothelial progenitor cells seeding heparin-coated decellularized scaffolds†
Article first published online: 24 NOV 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 100B, Issue 1, pages 111–120, January 2012
How to Cite
Zhou, M., Liu, Z., Liu, C., Jiang, X., Wei, Z., Qiao, W., Ran, F., Wang, W., Qiao, T. and Liu, C. (2012), Tissue engineering of small-diameter vascular grafts by endothelial progenitor cells seeding heparin-coated decellularized scaffolds. J. Biomed. Mater. Res., 100B: 111–120. doi: 10.1002/jbm.b.31928
- Issue published online: 6 DEC 2011
- Article first published online: 24 NOV 2011
- Manuscript Accepted: 25 JUN 2011
- Manuscript Revised: 22 MAY 2011
- Manuscript Received: 4 OCT 2010
- Important Science Project of Jiangsu Health. Grant Number: K200609
- tissue engineering vascular graft;
- endothelial progenitor cells;
- decellularized vessels;
- heparin coating
Successful construction of a small-diameter bioartificial vascular graft remains a great challenge. This study reports on novel tissue engineering vascular grafts (TEVGs) constructed by endothelial progenitor cells and heparin-coated decellularized vessels (DV). The DVs were fabricated from canine carotid arteries with observable depletion of cellular components. After heparin coating, the scaffolds possessed excellent antithrombogeneity. Canine endothelial progenitor cells harvested from peripheral blood were expanded and seeded onto heparin-coated DVs and cocultured in a custom-made bioreactor to construct TEVGs. Thereafter, the TEVGs were implanted into the carotid arteries of cell-donor dogs. After 3 months of implantation, the luminal surfaces of TEVGs exhibited complete endothelium regeneration, however, only a few disorderly cells and thrombosis overlaid the luminal surfaces of control DVs grafts, and immunofluorescent staining showed that the seeded cells existed in the luminal sides and the medial parts of the explanted TEVGs and partially contributed to the endothelium formation. Specifically, TEVGs exhibited significantly smaller hyperplastic neointima area compared with the DVs, not only at midportion (0.64 ± 0.08 vs. 2.13 ± 0.12 mm2, p < 0.001), but also at anastomotic sites (proximal sites, 1.03 ± 0.09 vs. 3.02 ± 0.16 mm2, p < 0.001; distal sites, 1.84 ± 0.15 vs. 3.35 ± 0.21 mm2, p < 0.001). Moreover, TEVGs had a significantly higher patency rate than the DVs after 3 months of implantation (19/20 vs. 12/20, p < 0.01). Overall, this study provided a new strategy to develop small-diameter TEVGs with excellent biocompatibility and high patency rate. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.