How to cite this article: Saha N, Dubey AK, Basu B. 2012. Cellular proliferation, cellular viability, and biocompatibility of HA-ZnO composites. J Biomed Mater Res Part B 2012:100B:256-264.
Article first published online: 21 NOV 2011
Copyright © 2011 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 100B, Issue 1, pages 256–264, January 2012
How to Cite
Saha, N., Dubey, A. K. and Basu, B. (2012), Cellular proliferation, cellular viability, and biocompatibility of HA-ZnO composites. J. Biomed. Mater. Res., 100B: 256–264. doi: 10.1002/jbm.b.31948
Laboratory for Biomaterials, Materials Research Centre, Indian Institute of Science (IISc), Bangalore, India
- Issue published online: 6 DEC 2011
- Article first published online: 21 NOV 2011
- Manuscript Accepted: 16 JUN 2011
- Manuscript Revised: 11 JUN 2011
- Manuscript Received: 22 OCT 2010
- hard tissue;
- cell-material interactions;
- cell proliferation
One of the important issues in the development of hydroxyapatite (HA)-based biomaterials is the prosthetic infection, which limits wider use of monolithic HA despite superior cellular response. Recently, we reported that ZnO addition to HA can induce bactericidal property. It is therefore important to assess how ZnO addition influences the cytotoxicity property and cell adhesion/proliferation on HA-ZnO composite surfaces in vitro. In the above perspective, the objective of this study is to investigate the cell type and material composition dependent cellular proliferation and viability of pressureless sintered HA-ZnO composites. The combination of cell viability data as well as morphological observations of cultured human osteoblast-like SaOS2 cells and mouse fibroblast L929 cells suggests that HA-ZnO composites containing 10 Wt % or lower ZnO exhibit the ability to support cell adhesion and proliferation. Both SaOS2 and L929 cells exhibit extensive multidirectional network of actin cytoskeleton and cell flattening on the lower ZnO containing (≤10 Wt %) HA-ZnO composites. The in vitro results illustrate how variation in ZnO content can influence significantly the cell vitality, as evaluated using MTT biochemical assay. Also, the critical statistical analysis reveals that ZnO addition needs to be carefully tailored to ensure good in vitro cytocompatibility. The underlying reasons for difference in biological properties are analyzed. It is suggested that surface wettability as well as dissolution of ZnO, both contribute to the observed differences in cellular viability and proliferation. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.