How to cite this article: Viola NV, Guerreiro-Tanomaru JM, da Silva GF, Sasso-Cerri E,Tanomaru-Filho M, Cerri PS. 2012. Biocompatibility of an experimental MTA Sealer implanted in the rat subcutaneous: quantitative and immunohistochemical evaluation. J Biomed Mater Res Part B 2012:100B:1773–1781.
Biocompatibility of an experimental MTA sealer implanted in the rat subcutaneous: Quantitative and immunohistochemical evaluation†
Article first published online: 23 JUL 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 100B, Issue 7, pages 1773–1781, October 2012
How to Cite
Viana Viola, N., Maria Guerreiro-Tanomaru, J., Ferreira da Silva, G., Sasso-Cerri, E., Tanomaru-Filho, M. and Cerri, P. S. (2012), Biocompatibility of an experimental MTA sealer implanted in the rat subcutaneous: Quantitative and immunohistochemical evaluation. J. Biomed. Mater. Res., 100B: 1773–1781. doi: 10.1002/jbm.b.32744
- Issue published online: 5 SEP 2012
- Article first published online: 23 JUL 2012
- Manuscript Accepted: 8 MAY 2012
- Manuscript Revised: 4 APR 2012
- Manuscript Received: 29 NOV 2011
- Fundação de Amparo à Pesquisa do Estado de São Paulo FAPESP. Grant Number: 2008/07455-5
- mineral trioxide aggregate;
- Portland cement;
- root canal sealer;
The tissue reaction promoted by an experimental mineral trioxide aggregate sealer (MTAS) in the rat subcutaneous was evaluated by morphological and morphometric analyses. In the animals from each group (n = 20), polyethylene tubes filled with MTAS, Portland cement (PC) or MTA were implanted in the dorsal subcutaneous. In the control group, empty tubes were implanted. After 7, 14, 30, and 60 days, the specimens were fixed and embedded in paraffin. In the HE-stained sections, the numerical density of inflammatory cells (IC) in the capsule was evaluated and statistical analyses performed (p ≤ 0.05). The expression of osteopontin (OPN) was evaluated by immunohistochemistry. The von Kossa method for detection of calcified structures was also performed. A moderate inflammatory process in the capsule was seen in all groups, at 7 and 14 days. At 60 days, significant reduction in the number of IC was verified in comparison to initial periods; however, significant differences were not verified among the groups. OPN immunolabeling was observed in the fibroblasts cytoplasm of the capsule next to the implants. Structures von Kossa-positive were observed in the capsule adjacent to all materials implanted at 7, 14, and 30 days. The results strongly indicate that MTAS presents biocompatibility similarly to MTA and PC. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.