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In vitro analysis of the cytotoxic and anti-inflammatory effects of antioxidant compounds used as additives in ultra high-molecular weight polyethylene in total joint replacement components†

  1. C. L. Bladen*,
  2. L. Tzu-Yin,
  3. J. Fisher‡,
  4. J. L. Tipper

Article first published online: 22 AUG 2012

DOI: 10.1002/jbm.b.32798

Issue

Journal of Biomedical Materials Research Part B: Applied Biomaterials

Journal of Biomedical Materials Research Part B: Applied Biomaterials

Volume 101B, Issue 3, pages 407–413, April 2013

Additional Information

How to Cite

Bladen, C. L., Tzu-Yin, L., Fisher, J. and Tipper, J. L. (2013), In vitro analysis of the cytotoxic and anti-inflammatory effects of antioxidant compounds used as additives in ultra high-molecular weight polyethylene in total joint replacement components. J. Biomed. Mater. Res., 101B: 407–413. doi: 10.1002/jbm.b.32798

Author Information

  1. Department of Biological Sciences, Institute of Medical and Biological Engineering, University of Leeds, LS29JT, UK

  1. ‡

    John Fisher is an NIHR senior investigator.

*Institute of Medical and Biological Engineering, University of Leeds, LS29JT, UK

  1. †

    How to cite this article: Bladen CL, Tzu-Yin L, Fisher J, Tipper JL. 2013. In vitro analysis of the cytotoxic and anti-inflammatory effects of antioxidant compounds used as additives in ultra high-molecular weight polyethylene in total joint replacement components. J Biomed Mater Res Part B 2013:101B:407–413.

  2. ‡

    John Fisher is an NIHR senior investigator.

Publication History

  1. Issue published online: 18 MAR 2013
  2. Article first published online: 22 AUG 2012
  3. Manuscript Accepted: 28 JUN 2012
  4. Manuscript Revised: 26 JUN 2012
  5. Manuscript Received: 12 JAN 2012

Funded by

  • WELMEC, a Centre of Excellence in Medical Engineering funded by the Wellcome Trust and EPSRC. Grant Number: WT 088908/Z/09/Z

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REFERENCES

  • 1
    Gomez-Barrena E, Puertolas JA, Munuera L, Konttinen YT. Update on UHMWPE research: From the bench to the bedside. Acta Orthop 2008; 79: 832–840.
  • 2
    Blunn G, Brach del Preva EM, Costa L, Fisher J, Freeman MA. Ultra high molecular-weight polyethylene (UHMWPE) in total knee replacement: Fabrication, sterilisation and wear. J Bone Joint Surg Br 2002; 84: 946–949.
  • 3
    Brach Del Prever EM, et al. UHMWPE for arthroplasty: past or future? J Orthop Traumatol 2009; 10: 1–8.
  • 4
    Sobieraj MC, Rimnac CM. Ultra high molecular weight polyethylene: mechanics, morphology, and clinical behavior. J Mech Behav Biomed Mater 2009; 2: 433–443.
  • 5
    Atwood SA, Van Citters DW, Patten EW, Furmanski J, Ries MD, Pruitt LA. Tradeoffs amongst fatigue, wear, and oxidation resistance of cross-linked ultra-high molecular weight polyethylene. J Mech Behav Biomed Mater 2011; 4: 1033–1045.
  • 6
    Kurtz SM, Ong KL, Lau E, Bozic KJ, Berry D, Parvizi J. Prosthetic joint infection risk after TKA in the Medicare population. Clin Orthop Relat Res 2010; 468: 52–56.
  • 7
    Oral E, Wannomae KK, Hawkins N, Harris WH, Muratoglu OK. Alpha-tocopherol-doped irradiated UHMWPE for high fatigue resistance and low wear. Biomaterials 2004; 25: 5515–5522.
  • 8
    Shibata N, Tomita N. The anti-oxidative properties of alpha-tocopherol in gamma-irradiated UHMWPE with respect to fatigue and oxidation resistance. Biomaterials 2005; 26: 5755–5762.
  • 9
    Oral E, Muratoglu OK. The effects of high dose irradiation on the cross-linking of vitamin E-blended ultrahigh molecular weight polyethylene. Biomaterials 2008; 29: 3557–3560.
  • 10
    Chumakov M, Silverman J, Al-Sheikhly M. Nitroxides as free radical scavengers in UHMWPE. In: 4th UHMWPE International Meeting Proceedings-UHMWPE for Arthroplasty: From powder to debris. Torino, Italy: UHMWPE lexicon; 2009.
  • 11
    Breese KD, Lamethe JF, Dearmitt C. Improving synthetic hindered phenol antioxidants: Learing from vitamin E. Polym Degrad Stab 2000; 70: 89–96.
  • 12
    Narayan VS, King R, Warner D, Sharpe M. Evaluation of antioxidant-stabilized UHMWPE materials. In: 55th Annual Meeting of the Orthopaedic Research Society. DePuy Orthopaedics Warsaw, IN, USA, Las Vegas, NV, 2009.
  • 13
    Lewinska A, Wnuk M, Slota E, Bartosz G. The nitroxide antioxidant tempol affects metal-induced cyto- and genotoxicity in human lymphocytes in vitro. Mutat Res 2008; 649: 7–14.
  • 14
    Palasz A, Czekaj P. Toxicological and cytophysiological aspects of lanthanides action. Acta Biochim Pol 2000; 47: 1108–1114.
  • 15
    Heng BC, Das GK, Zhao X, Ma LL, Tan TT, Ng KW, Loo JS. Comparative cytotoxicity evaluation of lanthanide nanomaterials on mouse and human cell lines with metabolic and DNA-quantification assays. Biointerphases 2010; 5: FA88–FA97.
  • 16
    Gallardo LA, Laurant M, Kunze J, Wimmer AMA. Lanthanides as stabilizing agents for UHMWPE. In: 4th UHMWPE International Proceedings-UHMWPE for Arthroplasty:from powder to debris. Torino, Italy: UHMWPE lexicon; 2009.
  • 17
    Forman HJ, Zhang H, Rinna A. Glutathione: Overview of its protective roles, measurement and biosynthesis. Mol Aspects Med 2008; 30: 1–12.
  • 18
    France R, Cidlowski JA. Apoptosis and glutathione: Beyond an antioxidant. Cell Death Differ 2009; 16: 1303–1314.
  • 19
    Malorni W, Iosi F, Santini MT, Testa U. Menadione-induced oxidative stress leads to a rapid down-modulation of transferrin receptor recycling. J Cell Sci 1993; 106( Pt 1): 309–318.
  • 20
    Laux I, Nel A. Evidence that oxidative stress-induced apoptosis by menadione involves Fas-dependent and Fas-independent pathways. Clin Immunol 2001; 101: 335–344.
  • 21
    Ingham E, Fisher J. Biological reactions to wear debris in total joint replacement. Proc Inst Mech Eng H 2000; 214: 21–37.
  • 22
    Teramura S, Russell S, Ingham E, Fisher J, Tomita N, Fujiwara K, Tipper JL. Reduced biological response to wear particles from UHMWPE containing vitamin E. In: 55th Annual Meeting of the Orthopaedic Research Society. University of Leeds Institute of Medical and Biological Engineering, Leeds, UK, Las Vegas, NV; 2009.
  • 23
    Bladen CL, Teramura S, Russell S, Fujiwara K, Fisher J, Ingham E, Tomita N, Tipper JL. Reduced inflammatory potential of vitamin E UHMWPR for use in total joint replacement. In: 5th UHMWPE International Meeting Proceedings-Advances in UHMWPE Research. Philadelphia, PA: UHMWPE lexicon; 2011.
  • 24
    Matthews JB, Green TR, Stone MH, Wroblewski BM, Fisher J, Ingham E. Comparison of the response of primary murine peritoneal macrophages and the U937 human histiocytic cell line to challenge with in vitro generated clinically relevant UHMWPE particles. Biomed Mater Eng 2000; 10: 229–240.
  • 25
    Oral E, Rowell SL, Muratoglu OK. The effect of alpha-tocopherol on the oxidation and free radical decay in irradiated UHMWPE. Biomaterials 2006; 27: 5580–5587.
  • 26
    Kurt SM, Muratoglu OK, Buchanan FJ, Currier B, Gsell R, Shen FW, Yau SS. Interlaboratory studies to determine optimal analytical methods for measuring the oxidation index of UHMWPE. Biomaterials 2001; 22: 2875–2881.
  • 27
    Gallardo LA, Carpentieri I, Laurent MP, Costa L, Wimmer MA. Effect of europium(II) stearate on the mechanical properties and the oxidation resistance of UHMWPE. J Mech Behav Biomed Mater 2011; 4: 821–826.
  • 28
    Oral E, Ghali BW, Muratoglu OK. The elimination of free radicals in irradiated UHMWPEs with and without vitamin E stabilization by annealing under pressure. J Biomed Mater Res B Appl Biomater 2011; 97: 167–174.
  • 29
    Gallardo LA, Knowlton CB, Kunze J, Jacobs JJ, Wimmer MA, Laurent MP. Europium stearate additives delay oxidation of UHMWPE for orthopaedic applications: A pilot study. Clin Orthop Relat Res 2011; 469: 2294–2301.
  • 30
    Teramura S, Sakoda H, Terao T, Endo MM, Fujiwara K, Tomita N. Reduction of wear volume from ultrahigh molecular weight polyethylene knee components by the addition of vitamin E. J Orthop Res 2008; 26: 460–464.
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  • 31
    Bracco P, Oral E. Vitamin E-stabilized UHMWPE for total joint implants: a review. Clin Orthop Relat Res 2011; 469: 2286–2293.
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