How to cite this article: Wang W, Cao B, Cui L, Cai J, Yin J. 2013. Adipose tissue engineering with human adipose tissue-derived adult stem cells and a novel porous scaffold. J Biomed Mater Res Part B 2013:101B:68–75.
Adipose tissue engineering with human adipose tissue-derived adult stem cells and a novel porous scaffold†
Article first published online: 22 OCT 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 101B, Issue 1, pages 68–75, January 2013
How to Cite
Wang, W., Cao, B., Cui, L., Cai, J. and Yin, J. (2013), Adipose tissue engineering with human adipose tissue-derived adult stem cells and a novel porous scaffold. J. Biomed. Mater. Res., 101B: 68–75. doi: 10.1002/jbm.b.32816
- Issue published online: 13 DEC 2012
- Article first published online: 22 OCT 2012
- Manuscript Accepted: 13 AUG 2012
- Manuscript Revised: 13 JUL 2012
- Manuscript Received: 11 FEB 2012
- tissue engineering;
- adipose tissue-derived adult stem cell;
- adipogenic differentiation;
- poly(L-glutamic acid);
- porous scaffolds
We investigated the effect of a novel porous scaffold composed with water-soluble poly(L-glutamic acid) (PLGA) and chitosan (CS) on the attachment, proliferation, and adipogenic differentiation of human adipose tissue-derived adult stem cells (ADSCs) in vitro and in vivo. Scanning electron microscope and fluorescent Dil labeling were used to reveal the attachment and growth of ADSCs on scaffolds; cell proliferation was detected by DNA assay. The adipogenic differentiation potential of ADSCs on the scaffolds was assayed by Oil-red O staining and further confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) for adipogenic gene markers (peroxisome proliferator-activated receptor γ2, lipoprotein lipase, fatty acid-binding protein, adiponectin). Cell-seeded constructs exposed to adipogenic medium for 2 weeks in vitro were implanted in severe combined immunodeficient (SCID) mice for 6 weeks. It was shown that ADSCs attached and spread well on scaffolds with good proliferation behaviors and abundance of extracellular matrix deposition. Oil-red O staining and RT-PCR showed adipogenic differentiation potential of ADSCs on scaffolds. Newly formed adipose-like tissue was confirmed in vivo in SCID mice by Oil-red O staining. PLGA/CS porous scaffolds exhibit good compatibility to ADSCs and can be promising biomaterials for adipose tissue engineering. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 68–75, 2013.