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Adipose tissue engineering with human adipose tissue-derived adult stem cells and a novel porous scaffold

Authors

  • Wei Wang,

    1. Department of Aesthetic and Plastic Surgery, The Second Hospital of Shandong University, Shandong University, Jinan, Shandong Province 250033, People's Republic of China
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  • Bin Cao,

    1. Department of Polymer Materials, Shanghai University, Jiading, Shanghai 201800, People's Republic of China
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  • Lei Cui,

    1. Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People's Republic of China
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  • Jinglong Cai,

    Corresponding author
    1. The 16th Department of Cosmetic Plastic Surgery, Plastic Surgery Hospital, Chinese Academy Medical Sciences, Peking Union Medical College, Beijing 100144, People's Republic of China
    • The 16th Department of Cosmetic Plastic Surgery, Plastic Surgery Hospital, Chinese Academy Medical Sciences, Peking Union Medical College, Beijing 100144, People's Republic of China
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  • Jingbo Yin

    Corresponding author
    1. Department of Polymer Materials, Shanghai University, Jiading, Shanghai 201800, People's Republic of China
    • Department of Polymer Materials, Shanghai University, Jiading, Shanghai 201800, People's Republic of China
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  • How to cite this article: Wang W, Cao B, Cui L, Cai J, Yin J. 2013. Adipose tissue engineering with human adipose tissue-derived adult stem cells and a novel porous scaffold. J Biomed Mater Res Part B 2013:101B:68–75.

Abstract

We investigated the effect of a novel porous scaffold composed with water-soluble poly(L-glutamic acid) (PLGA) and chitosan (CS) on the attachment, proliferation, and adipogenic differentiation of human adipose tissue-derived adult stem cells (ADSCs) in vitro and in vivo. Scanning electron microscope and fluorescent Dil labeling were used to reveal the attachment and growth of ADSCs on scaffolds; cell proliferation was detected by DNA assay. The adipogenic differentiation potential of ADSCs on the scaffolds was assayed by Oil-red O staining and further confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) for adipogenic gene markers (peroxisome proliferator-activated receptor γ2, lipoprotein lipase, fatty acid-binding protein, adiponectin). Cell-seeded constructs exposed to adipogenic medium for 2 weeks in vitro were implanted in severe combined immunodeficient (SCID) mice for 6 weeks. It was shown that ADSCs attached and spread well on scaffolds with good proliferation behaviors and abundance of extracellular matrix deposition. Oil-red O staining and RT-PCR showed adipogenic differentiation potential of ADSCs on scaffolds. Newly formed adipose-like tissue was confirmed in vivo in SCID mice by Oil-red O staining. PLGA/CS porous scaffolds exhibit good compatibility to ADSCs and can be promising biomaterials for adipose tissue engineering. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 68–75, 2013.

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