The anti-adhesive effect of thermally cross-linked gelatin film and its influence on the intestinal anastomosis in canine models

Authors

  • Hiroyuki Tsujimoto,

    Corresponding author
    1. Division of Medical Life System, Department of Life and Medical Science, Doshisha University, 1-3 Tatara-Miyakodani, Kyotanabe, Kyoto 610-0321, Japan
    • Division of Medical Life System, Department of Life and Medical Science, Doshisha University, 1-3 Tatara-Miyakodani, Kyotanabe, Kyoto 610-0321, Japan
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  • Ayumi Tanzawa,

    1. Division of Medical Life System, Department of Life and Medical Science, Doshisha University, 1-3 Tatara-Miyakodani, Kyotanabe, Kyoto 610-0321, Japan
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  • Mari Matoba,

    1. Division of Medical Life System, Department of Life and Medical Science, Doshisha University, 1-3 Tatara-Miyakodani, Kyotanabe, Kyoto 610-0321, Japan
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  • Ayumi Hashimoto,

    1. Division of Medical Life System, Department of Life and Medical Science, Doshisha University, 1-3 Tatara-Miyakodani, Kyotanabe, Kyoto 610-0321, Japan
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  • Shuko Suzuki,

    1. Medical Department, Nara Medical University, Kashihara, Nara 634-8521, Japan
    2. Research and Development Department, Gunze Ltd., Ayabe, Kyoto 623-8511, Japan
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  • Shinichiro Morita,

    1. Research and Development Department, Gunze Ltd., Ayabe, Kyoto 623-8511, Japan
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  • Yoshito Ikada,

    1. Medical Department, Nara Medical University, Kashihara, Nara 634-8521, Japan
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  • Akeo Hagiwara

    1. Division of Medical Life System, Department of Life and Medical Science, Doshisha University, 1-3 Tatara-Miyakodani, Kyotanabe, Kyoto 610-0321, Japan
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  • This paper has been written entirely by the authors, and no external funding was obtained for this study. The authors have no significant financial or any other relationship in this study.

  • How to cite this article: Matsuda T, Kuwana M, Aomizu T, Yamagishi M, Ohtake H, Watanabe G. 2013. Surface design for in situ capture of endothelial progenitor cells: VEGF-bound surface architecture and behaviors of cultured mononuclear cells. J Biomed Mater Res Part B 2013:101B:99–109.

Abstract

To generate a more effective and safer anti-adhesive material, we have developed a new type of thermally cross-linked gelatin film. In this study, we preclinically examined the anti-adhesive efficacy of this film and evaluated the possibility applying the film safely onto fresh intestinal anastomoses, compared with hyaluronate and carboxymethyl-cellulose (HA/CMC) film. Using a canine adhesion model, the degree of adhesion for each film was evaluated by adhesion scoring systems and histological observation. Three weeks after surgery, only the gelatin film showed significantly superior anti-adhesive effects compared to the control (no treatment), in particular, exhibiting excellent re-peritonization. Next, in a canine anastomosis model, the anastomoses were wrapped directly by each film and the bursting pressures of the anastomoses were examined 3 and 7 days after surgery. The gelatin film did not significantly affect either the bursting pressures or the healing process, compared with the control. However, the HA/CMC film significantly decreased the bursting pressures measured at 3 days after surgery. In conclusion, the thermally cross-linked gelatin film had satisfactory anti-adhesive effects with excellent re-peritonization. It could be safely applied to intestinal anastomoses without decreasing the bursting pressures. The gelatin film is considered to be quite favorable as an anti-adhesive material. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 99–109, 2013.

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