This paper has been written entirely by the authors, and no external funding was obtained for this study. The authors have no significant financial or any other relationship in this study.
Article first published online: 17 OCT 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 101B, Issue 1, pages 99–109, January 2013
How to Cite
Tsujimoto, H., Tanzawa, A., Matoba, M., Hashimoto, A., Suzuki, S., Morita, S., Ikada, Y. and Hagiwara, A. (2013), The anti-adhesive effect of thermally cross-linked gelatin film and its influence on the intestinal anastomosis in canine models . J. Biomed. Mater. Res., 101B: 99–109. doi: 10.1002/jbm.b.32821
How to cite this article: Matsuda T, Kuwana M, Aomizu T, Yamagishi M, Ohtake H, Watanabe G. 2013. Surface design for in situ capture of endothelial progenitor cells: VEGF-bound surface architecture and behaviors of cultured mononuclear cells. J Biomed Mater Res Part B 2013:101B:99–109.
- Issue published online: 13 DEC 2012
- Article first published online: 17 OCT 2012
- Manuscript Accepted: 20 AUG 2012
- Manuscript Revised: 21 JUL 2012
- Manuscript Received: 23 DEC 2011
- thermally cross-linked gelatin film;
- hyaluronate and carboxymethyl-cellulose film;
- intestinal anastomosis;
To generate a more effective and safer anti-adhesive material, we have developed a new type of thermally cross-linked gelatin film. In this study, we preclinically examined the anti-adhesive efficacy of this film and evaluated the possibility applying the film safely onto fresh intestinal anastomoses, compared with hyaluronate and carboxymethyl-cellulose (HA/CMC) film. Using a canine adhesion model, the degree of adhesion for each film was evaluated by adhesion scoring systems and histological observation. Three weeks after surgery, only the gelatin film showed significantly superior anti-adhesive effects compared to the control (no treatment), in particular, exhibiting excellent re-peritonization. Next, in a canine anastomosis model, the anastomoses were wrapped directly by each film and the bursting pressures of the anastomoses were examined 3 and 7 days after surgery. The gelatin film did not significantly affect either the bursting pressures or the healing process, compared with the control. However, the HA/CMC film significantly decreased the bursting pressures measured at 3 days after surgery. In conclusion, the thermally cross-linked gelatin film had satisfactory anti-adhesive effects with excellent re-peritonization. It could be safely applied to intestinal anastomoses without decreasing the bursting pressures. The gelatin film is considered to be quite favorable as an anti-adhesive material. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 99–109, 2013.