How to cite this article: Thanatvarakorn O., Nakashima S., Sadr A., Prasansuttiporn T., Ikeda M., Tagami J. 2013. In vitro evaluation of dentinal hydraulic conductance and tubule sealing by a novel calcium–phosphate desensitizer. J Biomed Mater Res Part B 2013:101B:303–309.
In vitro evaluation of dentinal hydraulic conductance and tubule sealing by a novel calcium–phosphate desensitizer†
Version of Record online: 19 NOV 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 101B, Issue 2, pages 303–309, February 2013
How to Cite
Thanatvarakorn, O., Nakashima, S., Sadr, A., Prasansuttiporn, T., Ikeda, M. and Tagami, J. (2013), In vitro evaluation of dentinal hydraulic conductance and tubule sealing by a novel calcium–phosphate desensitizer. J. Biomed. Mater. Res., 101B: 303–309. doi: 10.1002/jbm.b.32840
- Issue online: 8 JAN 2013
- Version of Record online: 19 NOV 2012
- Manuscript Accepted: 25 SEP 2012
- Manuscript Revised: 27 JUL 2012
- Manuscript Received: 14 MAY 2012
- dentin permeability
In the current trend of materials used for dentin hypersensitivity treatment, calcium–phosphate-containing desensitizers are expected to have advantages in oral environment. A newly formulated desensitizer containing tetracalcium phosphate and dicalcium phosphate anhydrous (CPD-100) was evaluated in comparison to oxalate containing desensitizer (SS) regarding permeability reduction (PR%) by measuring hydraulic conductance on the etched dentin discs in vitro. CPD-100 exhibited mean PR% of 91%, which significantly increased to 98% after immersion in artificial saliva (AS) for 4 weeks (p < 0.001), while SS showed a significant decrease from 99% to 93% (p < 0.01). SEM observation showed newly formed crystallites on CPD-100 treated dentin, which did not exist in SS treated dentin after AS immersion, suggesting that calcium oxalate inhibited formation of new calcium–phosphate minerals. Five-minute acid challenge did not significantly affect PR% of dentin treated by any of the desensitizers. The energy dispersive X-ray spectroscopy (EDS) analysis indicated that the formed layer of CPD-100 were minerals with similar Ca/P ratio to hydroxyapatite. In conclusion, the newly developed calcium–phosphate desensitizer has the potential to exhibit long-term stability in the oral environment, owing to its chemical properties that promote the crystal growth in salivary fluid. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.