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In vitro evaluation of dentinal hydraulic conductance and tubule sealing by a novel calcium–phosphate desensitizer

Authors

  • Ornnicha Thanatvarakorn,

    Corresponding author
    1. Cariology and Operative Dentistry, Department of Oral Health Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan
    2. Global Center Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan
    • Cariology and Operative Dentistry, Oral Restitution Department, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan
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  • Syozi Nakashima,

    1. Cariology and Operative Dentistry, Department of Oral Health Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan
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  • Alireza Sadr,

    1. Global Center Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan
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  • Taweesak Prasansuttiporn,

    1. Cariology and Operative Dentistry, Department of Oral Health Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan
    2. Global Center Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan
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  • Masaomi Ikeda,

    1. Department of Oral Health Care Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan
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  • Junji Tagami

    1. Cariology and Operative Dentistry, Department of Oral Health Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan
    2. Global Center Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan
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  • How to cite this article: Thanatvarakorn O., Nakashima S., Sadr A., Prasansuttiporn T., Ikeda M., Tagami J. 2013. In vitro evaluation of dentinal hydraulic conductance and tubule sealing by a novel calcium–phosphate desensitizer. J Biomed Mater Res Part B 2013:101B:303–309.

Abstract

In the current trend of materials used for dentin hypersensitivity treatment, calcium–phosphate-containing desensitizers are expected to have advantages in oral environment. A newly formulated desensitizer containing tetracalcium phosphate and dicalcium phosphate anhydrous (CPD-100) was evaluated in comparison to oxalate containing desensitizer (SS) regarding permeability reduction (PR%) by measuring hydraulic conductance on the etched dentin discs in vitro. CPD-100 exhibited mean PR% of 91%, which significantly increased to 98% after immersion in artificial saliva (AS) for 4 weeks (p < 0.001), while SS showed a significant decrease from 99% to 93% (p < 0.01). SEM observation showed newly formed crystallites on CPD-100 treated dentin, which did not exist in SS treated dentin after AS immersion, suggesting that calcium oxalate inhibited formation of new calcium–phosphate minerals. Five-minute acid challenge did not significantly affect PR% of dentin treated by any of the desensitizers. The energy dispersive X-ray spectroscopy (EDS) analysis indicated that the formed layer of CPD-100 were minerals with similar Ca/P ratio to hydroxyapatite. In conclusion, the newly developed calcium–phosphate desensitizer has the potential to exhibit long-term stability in the oral environment, owing to its chemical properties that promote the crystal growth in salivary fluid. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.

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