How to cite this article: Natarajan V, Krithica N, Madhan B, Sehgal PK. 2013. Preparation and properties of tannic acid cross-linked collagen scaffold and its application in wound healing. J Biomed Mater Res Part B 2013:101B:560–567.
Preparation and properties of tannic acid cross-linked collagen scaffold and its application in wound healing†
Article first published online: 19 DEC 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 101B, Issue 4, pages 560–567, May 2013
How to Cite
Natarajan, V., Krithica, N., Madhan, B. and Sehgal, P. K. (2013), Preparation and properties of tannic acid cross-linked collagen scaffold and its application in wound healing. J. Biomed. Mater. Res., 101B: 560–567. doi: 10.1002/jbm.b.32856
- Issue published online: 9 APR 2013
- Article first published online: 19 DEC 2012
- Manuscript Accepted: 5 OCT 2012
- Manuscript Revised: 30 AUG 2012
- Manuscript Received: 11 JAN 2012
- Council of Scientific and Industrial Research (CSIR) under Young Scientist Award (YSA) project scheme
- tannic acid;
- collagen scaffold;
- tissue engineering and regeneration;
- wound healing;
- enzymatic stability
A biodurable porous scaffold of collagen with good biocompatibility and enhanced wound healing potential is prepared through casting technique using tannic acid (TA) as crosslinker. The morphological analysis of the tannic acid cross-linked collagen scaffold (TCCs) distinctively shows scaly interlinks with large pores. The enzymatic stability of the scaffold is characterized in vitro to detail the role of TA in stabilization of collagen matrix against collagenolytic degradation. TCCs shows more stability (>54%) against collagenase than that of the collagen scaffolds (Cs). The attenuated total reflectance Fourier transform infrared analysis of the TCCs confirms the noncovalent interaction between collagen and TA. The biocompatibility of the scaffold (TCCs) in vitro has been established using 3T3 fibroblasts. Therapeutic and wound healing potential of the TCCs has been studied in vivo using excision wound model in rats. The results clearly indicates that the TCCs has greater and significant effect in wound closure and increased the wound healing rate compared with native Cs. This biocompatible and biodurable scaffold may find broad applications in the tissue engineering and drug delivery applications. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.