How to cite this article: Wolf-Brandstetter C, Roessler S, Storch S, Hempel U, Gbureck U, Nies B, Bierbaum S, Scharnweber D. 2013. Physicochemical and cell biological characterization of PMMA bone cements modified with additives to increase bioactivity. J Biomed Mater Res Part B 2013:101B:599–609.
Physicochemical and cell biological characterization of PMMA bone cements modified with additives to increase bioactivity†
Article first published online: 20 DEC 2012
Copyright © 2012 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 101B, Issue 4, pages 599–609, May 2013
How to Cite
Wolf-Brandstetter, C., Roessler, S., Storch, S., Hempel, U., Gbureck, U., Nies, B., Bierbaum, S. and Scharnweber, D. (2013), Physicochemical and cell biological characterization of PMMA bone cements modified with additives to increase bioactivity. J. Biomed. Mater. Res., 101B: 599–609. doi: 10.1002/jbm.b.32862
- Issue published online: 9 APR 2013
- Article first published online: 20 DEC 2012
- Manuscript Accepted: 16 OCT 2012
- Manuscript Revised: 11 SEP 2012
- Manuscript Received: 20 MAR 2012
- BMBF. Grant Number: 01EZ 0609
- DFG. Grant Number: TR79-B4
Additional Supporting Information may be found in the online version of this article.
|JBM_32862_sm_SuppFig1.tif||1403K||Supporting Information Figure 1. Additional mineral compounds observed with SEM on some spots of the surface after 7 days of incubation of PMMA cement with HEMA-P and 5% of both seeding salts (P/H/S5) in serum containing cell culture medium (crystal shape indicates the presence of calcite).|
|JBM_32862_sm_SuppFig2.tif||1408K||Supporting Information Figure 2. SEM image of PMMA cement with HEMA-P and 5% of both seeding salts (P/H/S5) after 7 days of incubation in simulated body fluid.|
|JBM_32862_sm_SuppFig3.tif||6086K||Supporting Information Figure 3. FTIR spectra of deposited mineral (analyzed after removal from differently modified PMMA cements incubated for 14 days either in A) cell culture medium with 15 % serum or B) simulated body fluid (black: reference spectrum of PMMA, blue: layer removed from reference cement P, red: layer removed from cement P/H, green: layer removed from cement P/H/S5), C) Reference spectra of hydroxyapatite (blue: thin HAp-layer on Ti6Al4V, green: commercially available HAp powder). Dashed line refers to one of the most typical bands for HAp at 1035 cm-1. As the FTIR spectra show mixed signals of the mineral layer and the PMMA bulk material, the exact nature of the deposited mineral cannot be identified clearly. However, compared to the reference spectrum of pure PMMA bulk material (black line in Fig S3A and S3B) which shows no peak at 1035 cm-1, the spectra for the minerals obtained from all samples after incubation in cell culture medium clearly show a higher signal in this region, most pronounced in the case of P/H/S5 due to the higher available amount of mineral in relation to the bulk material.|
|JBM_32862_sm_SuppFig4.tif||312K||Supporting Information Figure 4. DNA content of hMSC seeded on different cement types after 7 d of pre-incubation in cell culture medium (mean of 2 donors, each with n=4). Two way anova analysis: significant differences in cell behaviour between the different substrates are indicated with asterisks besides the legend.|
|JBM_32862_sm_SuppFig5.tif||419K||Supporting Information Figure 5. Activity of alkaline phosphatase (normalized to individual DNA content of the lysates) for cells grown on different substrates after 7 d of pre-incubation, n=4.|
|JBM_32862_sm_SuppFig6.tif||29452K||Supporting Information Figure 6. Surface energy of different cement preparations, calculated by SPA software (Dataphysics, Germany) using the OWRK model after analysis of contact angles with water and methylene iodide with the sessile drop method.|
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