Both authors contributed equally to this work.
Effects of different sterilization techniques and varying anodized TiO2 nanotube dimensions on bacteria growth†
Article first published online: 29 JAN 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 101B, Issue 5, pages 677–688, July 2013
How to Cite
Kummer, K. M., Taylor, E. N., Durmas, N. G., Tarquinio, K. M., Ercan, B. and Webster, T. J. (2013), Effects of different sterilization techniques and varying anodized TiO2 nanotube dimensions on bacteria growth. J. Biomed. Mater. Res., 101B: 677–688. doi: 10.1002/jbm.b.32870
How to cite this article: Kummer KM, Taylor EN, Durmas NG, Tarquinio KM, Ercan B, Webster TJ. 2013. Effects of different sterilization techniques and varying anodized TiO2 nanotube dimensions on bacteria growth. J Biomed Mater Res Part B 2013:101B:677–688.
- Issue published online: 8 JUN 2013
- Article first published online: 29 JAN 2013
- Manuscript Accepted: 29 OCT 2012
- Manuscript Revised: 23 OCT 2012
- Manuscript Received: 6 JUL 2012
- bacterial adherence;
- nanomodified surfaces;
- titanium (alloys)
Infection of titanium (Ti)-based orthopedic implants is a growing problem due to the ability of bacteria to develop a resistance to today's antibiotics. As an attempt to develop a new strategy to combat bacteria functions, Ti was anodized in the present study to possess different diameters of nanotubes. It is reported here for the first time that Ti anodized to possess 20 nm tubes then followed by heat treatment to remove fluorine deposited from the HF anodization electrolyte solution significantly reduced both S. aureus and S. epidermidis growth compared to unanodized Ti controls. It was further found that the sterilization method used for both anodized nanotubular Ti and conventional Ti played an important role in the degree of bacteria growth on these substrates. Overall, UV light and ethanol sterilized samples decreased bacteria growth, while autoclaving resulted in the highest amount of bacteria growth. In summary, this study indicated that through a simple and inexpensive process, Ti can be anodized to possess 20 nm tubes that no matter how sterilized (UV light, ethanol soaking, or autoclaving) reduces bacteria growth and, thus, shows great promise as an antibacterial implant material. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part 2013.