How to cite this article: Khojasteh A, Behnia H, Hosseini FS, Dehghan MM, Abbasnia P, Abbas FM. 2013. The effect of PCL-TCP scaffold loaded with mesenchymal stem cells on vertical bone augmentation in dog mandible: A preliminary report. J Biomed Mater Res Part B 2013:101B:848–854.
The effect of PCL-TCP scaffold loaded with mesenchymal stem cells on vertical bone augmentation in dog mandible: A preliminary report†
Article first published online: 29 JAN 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 101B, Issue 5, pages 848–854, July 2013
How to Cite
Khojasteh, A., Behnia, H., Hosseini, F. S., Dehghan, M. M., Abbasnia, P. and Abbas, F. M. (2013), The effect of PCL-TCP scaffold loaded with mesenchymal stem cells on vertical bone augmentation in dog mandible: A preliminary report. J. Biomed. Mater. Res., 101B: 848–854. doi: 10.1002/jbm.b.32889
- Issue published online: 8 JUN 2013
- Article first published online: 29 JAN 2013
- Manuscript Accepted: 29 NOV 2012
- Manuscript Revised: 20 NOV 2012
- Manuscript Received: 27 APR 2012
- Dental research Center, Research Institute of Dental Sciences, Faculty of Dentistry, Shahid Beheshti University of Medical Sciences
- bone engineering;
- mesenchymal stem cell;
- biodegradable polymers;
- bone regeneration
Polycaprolactone-tricalcium phosphate (PCL-TCP), a new composite scaffold, has been shown to facilitate early revascularization and speed up bone regeneration process. The objective of this study was to evaluate the effect of PCL-TCP seeded with mesenchymal stem cells (MSCs) on healing of the vertical bone critical sized defect in dog's mandible. Bone marrow aspirate from dog humerous was cultured and the stemness of the cells was examined by differentiation staining methods and flow cytometric analysis. Third passage subculture cells (5 × 105 cells) were loaded on 20 × 10 × 10 mm3 and incubated for 48 h. The presence of MSCs in the pores was evaluated by scanning electron microscope. Bilateral mandibular premolar teeth were extracted in four dogs and the buccal and lingual bone plates were reduced to make a vertical defect. Cell-loaded scaffolds were fixed in right side and left side received pure PCL-TCP scaffolds as a control side defects. Histomorphometric analysis after 8 weeks of the scaffold implantation showed higher amount of lamellar bone in the test side (48.63%) than control side (17.27%) (p < 0.05).The results suggest that PCL-TCP may be an appropriate scaffold for loading MSCs in bone regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.