How to cite this article: Avci-Adali M, Kobba J, Neumann B, Lescan M, Perle N, Wilhelm N, Wiedmaier H, Schlensak C, Wendel HP. 2013. Application of a rotating bioreactor consisting of low-cost and ready-to-use medical disposables for in vitro evaluation of the endothelialization efficiency of small-caliber vascular prostheses. J Biomed Mater Res Part B 2013:101B:1061–1068.
Application of a rotating bioreactor consisting of low-cost and ready-to-use medical disposables for in vitro evaluation of the endothelialization efficiency of small-caliber vascular prostheses†
Article first published online: 7 MAY 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 101B, Issue 6, pages 1061–1068, August 2013
How to Cite
Avci-Adali, M., Kobba, J., Neumann, B., Lescan, M., Perle, N., Wilhelm, N., Wiedmaier, H., Schlensak, C. and Wendel, H. P. (2013), Application of a rotating bioreactor consisting of low-cost and ready-to-use medical disposables for in vitro evaluation of the endothelialization efficiency of small-caliber vascular prostheses. J. Biomed. Mater. Res., 101B: 1061–1068. doi: 10.1002/jbm.b.32916
- Issue published online: 12 JUL 2013
- Article first published online: 7 MAY 2013
- Manuscript Accepted: 30 JAN 2013
- Manuscript Revised: 30 NOV 2012
- Manuscript Received: 8 JUN 2012
- European Social Funds in Baden-Wuerttemberg, Germany
- German Federal Ministry of Education and Research (BMBF)
- vascular prostheses;
- rotating bioreactor;
- endothelial cell seeding
The incomplete endothelialization of especially small-caliber vascular prostheses after implantation in patients is a major disadvantage in cardiovascular interventions. The lack of an endothelium leads to the occurrence of thrombosis at the luminal surface of artificial vascular prostheses. Thus, the development of new graft materials and coatings for induction of complete endothelialization on the implant surfaces is a promising approach to improve hemocompatibility and maintain long-term graft patency. In this study, we designed a rotation model to evaluate the early endothelial cell (EC) seeding efficiency of different small-caliber vascular devices, such as stents and vascular grafts. The suitability of the designed rotation model for endothelialization studies was investigated by seeding and cultivation of prostheses with ECs followed by scanning electron microscopy. Furthermore, the viability of attached ECs was determined by calcein acetoxymethyl ester (AM) staining. The rotation model consisting of low-cost medical disposables enabled sterile incubation and cultivation of ECs with vascular devices. Simultaneously, the rotation of the bioreactor ensured a uniform distribution and adhesion of cells to the devices. Calcein AM staining of adherent cells on prostheses revealed excellent cell viability. Moreover, using the designed rotation model, an influence of different coatings and materials on the adhesion and spreading of ECs was demonstrated. The rotating bioreactor described and used in this study not only saves time and money but is also eminently useful for the accelerated preclinical evaluation of the endothelialization efficiency of different materials and surface coatings of small-caliber vascular devices. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.