How to cite this article: Son S-R, Franco R-A, Bae S-H, Min Y-K, Lee B-T. 2013. Electrospun PLGA/gelatin fibrous tubes for the application of biodegradable intestinal stent in rat model. J Biomed Mater Res Part B 2013:101B:1095–1105.
Electrospun PLGA/gelatin fibrous tubes for the application of biodegradable intestinal stent in rat model†
Article first published online: 6 APR 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 101B, Issue 6, pages 1095–1105, August 2013
How to Cite
Son, S.-R., Franco, R.-A., Bae, S.-H., Min, Y.-K. and Lee, B.-T. (2013), Electrospun PLGA/gelatin fibrous tubes for the application of biodegradable intestinal stent in rat model. J. Biomed. Mater. Res., 101B: 1095–1105. doi: 10.1002/jbm.b.32923
- Issue published online: 12 JUL 2013
- Article first published online: 6 APR 2013
- Manuscript Accepted: 11 FEB 2013
- Manuscript Revised: 10 JAN 2013
- Manuscript Received: 13 OCT 2012
- Korea Health Technology R&D project, Ministry of Health & Welfare, Republic of Korea
- intestinal stent
A biodegradable fibrous tube was fabricated by electrospinning method using a combination of Poly(lactic-co-glycolic acid) (PLGA) and gelatin dissolved in trifluoroethanol (TFE). Different ratios of the two polymers (PLGA/Gelatin: 1/9, 3/7, 5/5) were used for electrospinning to determine the optimum condition appropriate for intestinal stent application. Fiber morphology was visualized and analyzed using a scanning electron microscope (SEM). Characterizations of physical properties were done according to its tensile strength, surface hydrophilicity, swelling ability, and biodegradability. Biocompatibility of the scaffolds was investigated in vitro using IEC-18 (Rat intestinal epithelial cell). Cell proliferation was quantified using MTT assay and cell adhesion behavior was visualized by SEM and confocal laser scanning microscope. PLGA/Gelatin (5/5) was determined to have adequate material properties and sufficient in vitro biocompatibility. This was then implanted in a male Sprague-Dawley rat for 14 days to determine in vivo behavior of the sample. Histological examination on the intestinal tissue surrounding the graft showed normal morphology comparable to non-implanted intestine. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.