This work is dedicated to Dr. Heike Hall. *Both authors contributed equally to this work. †Deceased.
Original Research Report
Influence of micro and submicro poly(lactic-glycolic acid) fibers on sensory neural cell locomotion and neurite growth
Article first published online: 7 MAY 2013
Copyright © 2013 Wiley Periodicals, Inc., a Wiley Company
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 101, Issue 7, pages 1200–1208, October 2013
How to Cite
How to cite this article: 2013. Influence of micro and submicro poly(lacticglycolic acid) fibers on sensory neural cell locomotion and neurite growth. J Biomed Mater Res Part B 2013:101B:1200–1208., , , , .
- Issue published online: 11 SEP 2013
- Article first published online: 7 MAY 2013
- Manuscript Accepted: 17 FEB 2013
- Manuscript Revised: 11 FEB 2013
- Manuscript Received: 2 NOV 2012
- Competence Center for Materials Science and Technology CCMX MatLife. Grant Number: 0-21154-07
- cell-material interactions;
- nerve regeneration;
- neurite alignment
For successful peripheral nerve regeneration, a complex interplay of growth factors, topographical guidance structure by cells and extracellular matrix proteins, are needed. Aligned fibrous biomaterials with a wide variety in fiber diameter have been used successfully to support neuronal guidance. To better understand the importance of size of the topographical features, we investigated the directionality of neuronal migration of sensory ND7/23 cells on aligned electrospun poly(lactic-glycolic acid) PLGA fibers in the range of micrometer and submicrometer diameters by time-lapse microscopy. Cell trajectories of single ND7/23 cells were found to significantly follow topographies of PLGA fibers with micrometer dimensions in contrast to PLGA fibers within the submicrometer range, where cell body movement was observed to be independent of fibrous structures. Moreover, neurite alignment of ND7/23 cells on various topographies was assessed. PLGA fibers with micrometer dimensions significantly aligned 83.3% of all neurites after 1 day of differentiation compared to similar submicrometer structures, which orientated 25.8% of all neurites. Interestingly, after 7 days of differentiation ND7/23 cells on submicrometer PLGA fibers increased their alignment of neurites to 52.5%. Together, aligned PLGA fibers with micrometer dimensions showed a superior influence on directionality of neuronal migration and neurite outgrowth of sensory ND7/23 cells, indicating that electrospun micro-PLGA fibers might represent a potential material to induce directionality of neuronal growth in engineering applications for sensory nerve regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 1200–1208, 2013.