Quantitation of cholesterol and phospholipid sorption on silicone hydrogel contact lenses
Version of Record online: 7 JUN 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 101, Issue 8, pages 1516–1523, November 2013
How to Cite
How to cite this article: 2013. Quantitation of cholesterol and phospholipid sorption on silicone hydrogel contact lenses. J Biomed Mater Res Part B 2013:101B:1516–1523., , , , , , .
- Issue online: 22 OCT 2013
- Version of Record online: 7 JUN 2013
- Manuscript Accepted: 21 APR 2013
- Manuscript Revised: 29 MAR 2013
- Manuscript Received: 8 NOV 2012
- Bausch and Lomb, Inc.
- and the Pope Professorship of Brigham Young University
- silicone hydrogel contact lens;
- lipid sorption;
The introduction of silicone hydrogel (SiHy) contact lenses to the consumer marketplace necessitates study of the susceptibility of these lenses to spontaneous deposition by hydrophobic lipid components of ocular tears. The use of radioisotopes to measure lipid sorption on SiHy contact lenses gives precise and accurate results but requires institutional infrastructure and compels efficient lipid removal from the lens. This study compares three methods of quantitating phospholipid and cholesterol sorption on SiHy lenses using radiolabeled cholesterol and phosphatidylcholine that were sorbed on lenses from an artificial tear fluid. A triple extraction technique using n-propanol gives the most reliable results. Comparison of sorption on SiHy lenses shows that balafilcon A and senofilcon A lenses sorb similar amounts, while lotrafilcon B lenses sorb comparatively less. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 101B: 1516–1523, 2013.