Original Research Report
Photocatalytic inactivation of biofilms on bioactive dental adhesives
Article first published online: 11 JUL 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 102, Issue 1, pages 62–67, January 2014
How to Cite
How to cite this article: 2014. Photocatalytic inactivation of biofilms on bioactive dental adhesives. J Biomed Mater Res Part B 2014:102B: 62–67., , , , .
- Issue published online: 10 DEC 2013
- Article first published online: 11 JUL 2013
- Manuscript Accepted: 5 MAY 2013
- Manuscript Revised: 27 APR 2013
- Manuscript Received: 11 DEC 2012
- The Carl Trygger Foundation, The Göran Gustafsson Foundation, The Swedish Research Council, Vinnova, and The Swedish Foundation for Strategic Research
- titanium dioxide;
- biofilm inactivation;
- Streptococcus mutans;
- metabolic activity assay
Biofilms are the most prevalent mode of microbial life in nature and are 10–1000 times more resistant to antibiotics than planktonic bacteria. Persistent biofilm growth associated at the margin of a dental restoration often leads to secondary caries, which remains a challenge in restorative dentistry. In this work, we present the first in vitro evaluation of on-demand photocatalytic inactivation of biofilm on a novel dental adhesive containing TiO2 nanoparticles. Streptococcus mutans biofilm was cultured on this photocatalytic surface for 16 h before photocatalytic treatment with ultraviolet-A (UV-A) light. UV-A doses ranging from 3 to 43 J/cm2 were applied to the surface and the resulting viability of biofilms was evaluated with a metabolic activity assay incorporating phenol red that provided a quantitative measure of the reduction in viability due to the photocatalytic treatments. We show that an UV-A irradiation dose of 8.4 J/cm2 leads to one order of magnitude reduction in the number of biofilm bacteria on the surface of the dental adhesives while as much as 5–6 orders of magnitude reduction in the corresponding number can be achieved with a dose of 43 J/cm2. This material maintains its functional properties as an adhesive in restorative dentistry while offering the possibility of a novel dental procedure in the treatment or prevention of bacterial infections via on-demand UV-A irradiation. Similar materials could be developed for the treatment of additional indications such as peri-implantits. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 62–67, 2014.