Original Research Report
Three-dimensional configuration of orientated fibers as guidance structures for cell migration and axonal growth
Version of Record online: 16 SEP 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 102, Issue 2, pages 356–365, February 2014
How to Cite
How to cite this article: 2014. Three-dimensional configuration of orientated fibers as guidance structures for cell migration and axonal growth. J Biomed Mater Res Part B 2014: 102B:356–365., , , , , .
- Issue online: 11 JAN 2014
- Version of Record online: 16 SEP 2013
- Manuscript Accepted: 29 JUL 2013
- Manuscript Revised: 11 JUL 2013
- Manuscript Received: 7 MAY 2013
- Deutsche Forschungsgemeinschaft . Grant Number: ME 1261/11 (to J.M.)
- peripheral nerve;
- artificial implants;
- extracellular matrix;
- growth factors
Peripheral nerve injuries can be surgically repaired by suturing the transected nerve stumps or, in case of larger lesions, by the transplantation of an autologous nerve graft. To avoid donor site morbidity, the development of artificial implants is desired. Clinically, hollow conduits have been used for this purpose but are inferior to the autograft because they lack internal guidance cues for Schwann cells and regenerating axons. In this article, we describe the design of a three-dimensional (3D) scaffold consisting of parallel fibers embedded in a collagen matrix. For this purpose, an electrospinning device was developed to produce and manipulate a 3D array of aligned poly(ɛ-caprolactone) (PCL) microfibers. This fiber array was then incorporated into biodegradable PCL tubes to serve as artificial nerve bridges. Using primary cultures of embryonic chicken dorsal root ganglia, we show that PCL microfibers in the 3D matrix of our composite scaffold guide the direction of Schwann cell migration and axonal growth. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 356–365, 2014.