Original Research Report
Three-dimensional collagen scaffold enhances the human adenoid cystic carcinoma cancer stem cell and epithelial–mesenchymal transition properties
Article first published online: 21 OCT 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 102, Issue 4, pages 772–780, May 2014
How to Cite
How to cite this article: 2014. Three-dimensional collagen scaffold enhances the human adenoid cystic carcinoma cancer stem cell and epithelial–mesenchymal transition properties. J Biomed Mater Res Part B 2014: 102B: 772–780., , , , , , .
- Issue published online: 11 APR 2014
- Article first published online: 21 OCT 2013
- Manuscript Accepted: 27 SEP 2013
- Manuscript Revised: 28 AUG 2013
- Manuscript Received: 17 JUN 2013
- Ministry of Science and Technology of China . Grant Number: 2010CB529403 and 2011CB965001
- National Natural Science Foundation of China . Grant Number: 81072230
- adenoid cystic carcinoma;
- three-dimensional culture;
- collagen scaffold;
- cancer stem cell;
- mesenchymal transition
Three-dimensional (3D) cell culture may provide the architectures similar to the in vivo natural extracellular matrix condition for in vitro cultured cells. In this work, a 3D collagen scaffold was used to culture the adenoid cystic carcinoma (ACC) cells. The results showed that the 3D scaffold not only induced the diversification of cell morphologies but also increased the cell proliferation. The transcription of matrix metalloproteinase and epithelial–mesenchymal transition were significantly increased in the cells cultured in 3D collagen scaffolds. In addition, the expression of cancer stem cell (CSC) markers of Sox2 and Oct4 were higher than that in 2D cultured cells. The 3D cultured ACC-83 cells showed more resistance to chemotherapeutic drugs. Thus, the 3D collagen scaffold could provide a useful model for CSCs study and anticancer therapeutics research in vitro. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 772–780, 2014.