Original Research Report
Distribution of molecules locally delivered from bone cement
Article first published online: 24 OCT 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Biomedical Materials Research Part B: Applied Biomaterials
Volume 102, Issue 4, pages 806–814, May 2014
How to Cite
How to cite this article: 2014. Distribution of molecules locally delivered from bone cement. J Biomed Mater Res Part B 2014: 102B: 806–814., , , , .
- Issue published online: 11 APR 2014
- Article first published online: 24 OCT 2013
- Manuscript Accepted: 27 SEP 2013
- Manuscript Revised: 23 SEP 2013
- Manuscript Received: 1 JUL 2013
- Arizona Biomedical Research Commission . Grant Number: 1116
- Banner Good Samaritan Medical Center, Herbert Louis Fund at Orthopaedic Research and Education Foundation, Ira A. Fulton Schools of Engineering (to M.B.G.) and Achievement Rewards for College Scientists
- drug delivery;
- medical imaging;
- controlled release;
- bone cement
Revision of infected orthopedic implants is successful in most cases when antimicrobials are delivered locally (mixed with bone cement or bone graft which is placed in the site from which the infected tissue was removed); however, there is still a substantial rate of recurrence most likely due to the antimicrobials not achieving a therapeutic dose at all locations in the tissue. To study transport within this environment, gadolinium chelated in diethylene triamine pentaacetic acid (Gd-DTPA), a MRI contrast agent with size and solubility similar to two common antimicrobials (gentamicin and vancomycin), was mixed with bone cement, implanted in vivo into two models of orthopedic surgical wounds, and imaged using MRI 5.5 h after implantation. Image thresholding was used to create two-dimensional and three-dimensional representations of areas/volumes containing detectable concentrations of Gd-DTPA. Distribution is found to be anisotropic with Gd-DTPA transporting preferentially anterior of the implant toward the skin. When fascia is not closed over the implant site, Gd-DTPA transports to the skin and along the subcutaneous plane. The distance transported indicates that transport is likely driven by convection. Finally, the tissue concentration of Gd-DTPA is much less than the concentration loaded into the bone cement. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 806–814, 2014.