MFM and JEA contributed equally to this work.
The R740S mutation in the V-ATPase a3 subunit increases lysosomal pH, impairs NFATc1 translocation, and decreases in vitro osteoclastogenesis
Article first published online: 18 DEC 2012
Copyright © 2013 American Society for Bone and Mineral Research
Journal of Bone and Mineral Research
Volume 28, Issue 1, pages 108–118, January 2013
How to Cite
Voronov, I., Ochotny, N., Jaumouillé, V., Owen, C., Manolson, M. F. and Aubin, J. E. (2013), The R740S mutation in the V-ATPase a3 subunit increases lysosomal pH, impairs NFATc1 translocation, and decreases in vitro osteoclastogenesis. J Bone Miner Res, 28: 108–118. doi: 10.1002/jbmr.1727
- Issue published online: 18 DEC 2012
- Article first published online: 18 DEC 2012
- Accepted manuscript online: 3 AUG 2012 10:33AM EST
- Manuscript Accepted: 16 JUL 2012
- Manuscript Revised: 7 JUN 2012
- Manuscript Received: 10 JAN 2012
Additional Supporting Information may be found in the online version of this article.
|jbmr_1727_sm_SupplFig1.tif||230K||Supplementary Figure 1. Effect of lysosomal inhibitors on osteoclast viability. Briefly, osteoclasts were cultured as described in Methods; inhibitors (ammonium chloride, chloroqine, concanamycin A) were added on day 5 and cells incubated for 24 hrs. Cell viability was assessed using an XTT-based Cell Proliferation kit (FroggaBio) following the manufacturer's instructions.|
|jbmr_1727_sm_SupplTab1.doc||32K||Supplementary Table 1|
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