WFX, JUJ, CS, and SX contributed equally to this work.
Swedish mutant APP suppresses osteoblast differentiation and causes osteoporotic deficit, which are ameliorated by N-acetyl-L-cysteine
Article first published online: 18 SEP 2013
© 2013 American Society for Bone and Mineral Research
Journal of Bone and Mineral Research
Volume 28, Issue 10, pages 2122–2135, October 2013
How to Cite
Xia, W.-F., Jung, J.-U., Shun, C., Xiong, S., Xiong, L., Shi, X.-M., Mei, L. and Xiong, W.-C. (2013), Swedish mutant APP suppresses osteoblast differentiation and causes osteoporotic deficit, which are ameliorated by N-acetyl-L-cysteine. J Bone Miner Res, 28: 2122–2135. doi: 10.1002/jbmr.1954
- Issue published online: 18 SEP 2013
- Article first published online: 18 SEP 2013
- Accepted manuscript online: 3 MAY 2013 07:12AM EST
- Manuscript Accepted: 25 MAR 2013
- Manuscript Revised: 8 MAR 2013
- Manuscript Received: 3 DEC 2012
- National Institutes of Health (to WCX and LM)
- U.S. Veteran's Administration (WCX)
Additional Supporting Information may be found in the online version of this article.
|jbmr1954-sm-0001-SupFig-S1.pdf||1487K||Supplement Fig. 1. Generation of the conditional transgenic mice selectively expressing APPswe in a manner dependent on Cre-recombinase. (A) Illustration of the transgene plasmid and the breeding plan. (B) Genotyping results of the transgenic mice. (C) Western blot analysis of APPswe expression in muscle homogenates derived from TgAPPswe-HSA germ-line pups. Note that APPswe expression was induced when HAS-Cre was present. The founder line #3 was chosen for further breeding with Ocn-Cre to generate TgAPPswe-Ocn mice. (D) Images of the Tg(flox)APPswe and TgAPPswe-Ocn mice. (E) Reduced body weight was detected in TgAPPswe-Ocn mice.|
|jbmr1954-sm-0002-SupFig-S2.jpg||478K||Supplement Fig. 2. Expression of endogenous APP and APPswe in BMSCs. (A) A representative phase contrast image of purified BMSCs from 18-month old mice (C57BL/6). (B) Western blot analysis for endogenous APP expression in indicated aged BMSCs using indicated antibodies. (C) Western blot analysis for APP and APPswe expression in BMSCs and BMMs derived from control and TgAPPswe-Ocn mice (at age of 3-month old).|
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