TZ and FC are co-first authors.
The G60S connexin 43 mutation activates the osteoblast lineage and results in a resorption-stimulating bone matrix and abrogation of old-age–related bone loss
Article first published online: 18 OCT 2013
© 2013 American Society for Bone and Mineral Research
Journal of Bone and Mineral Research
Volume 28, Issue 11, pages 2400–2413, November 2013
How to Cite
Zappitelli, T., Chen, F., Moreno, L., Zirngibl, R. A., Grynpas, M., Henderson, J. E. and Aubin, J. E. (2013), The G60S connexin 43 mutation activates the osteoblast lineage and results in a resorption-stimulating bone matrix and abrogation of old-age–related bone loss. J Bone Miner Res, 28: 2400–2413. doi: 10.1002/jbmr.1965
- Issue published online: 18 OCT 2013
- Article first published online: 18 OCT 2013
- Accepted manuscript online: 19 APR 2013 07:10AM EST
- Manuscript Accepted: 15 APR 2013
- Manuscript Revised: 8 APR 2013
- Manuscript Received: 12 NOV 2012
- Canadian Institutes of Health Research (CIHR) operating grant (FRN 69198 to JEA) and the Quebec Transgenic Research Network (to JEH), as well as scholarship support from CIHR-Skeletal Regenerative Medicine Team (to TZ), Queen Elizabeth II-GSST (to TZ); Department of Medical Biophysics (University of Toronto) (to TZ); Canadian Arthritis Network (to RAZ), and CIHR/Osteoporosis Society (to RAZ)
Additional Supporting Information may be found in the online version of this article.
Supplemental Fig. 1. Longitudinal analysis of cortical parameters. (A) Histomorphometric analysis of the structural properties of the femurs showed significantly decreased cortical bone area at all ages and decreased marrow area at 4- 12 months in Gja1Jrt/+ versus WT mice. Solid and dashed lines indicate significant differences over time in WT and Gja1Jrt/+ mice, respectively; n ≥ 6. (B) There was no difference in the percentage of lacunae that were empty in the cortical bone of Gja1Jrt/+ versus WT mice at 2 and 8 months of age; n ≥ 4. *p < 0.05, **p < 0.01 and ***p < 0.001.
Supplemental Fig. 2. Analysis of osteoblast and osteocyte-specific genes in cortical bone extracts. (A) Expression of early-, mid- and late-osteoblast and osteocyte differentiation markers were unaffected in RNA isolated from cortical bone of 2, 4, 8 and 12 month old mice, with the exception of decreased Sost expression at 2 months in Gja1Jrt/+ versus WT mice; n ≥ 3 and each sample is comprised of two or more independent biological samples. Samples were run in triplicate. *p < 0.05, **p < 0.01 and ***p < 0.001.
Supplemental Fig. 3. Expression of Rankl-Opg and osteoclast-specific gene expression in Gja1Jrt/+ versus WT mice. (A) The Rankl/Opg expression ratio in RNA extracted from cortical bone at all ages was unaffected in Gja1Jrt/+ versus WT; n ≥ 3 and each sample is comprised of two or more independent biological samples. (B) Rankl/Opg expression ratio was also unaffected in trabecular bone at 2 and 4 months of age; n ≥ 4. (C) Expression of osteoclast differentiation and fusion markers was unaffected in RNA from trabecular bone of 2 and 4 month-old Gja1Jrt/+ versus WT mice; n ≥ 3. Samples were run in triplicate. *p < 0.05, **p < 0.01 and ***p < 0.001.
Supplemental Table 1. Quantitative RT-PCR primer sequences used in this study.
Supplemental Table 2. List of antibodies used in this study.
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