Sarcopenia is associated with a greater fracture risk. This relationship was originally thought to be explained by an increased risk of falls in sarcopenic individuals. However, in addition, there is growing evidence of a functional muscle-bone unit in which bone health may be directly influenced by muscle function. Because a definition of sarcopenia encompasses muscle size, strength, and physical performance, we investigated relationships for each of these with bone size, bone density, and bone strength to interrogate these hypotheses further in participants from the Hertfordshire Cohort Study. A total of 313 men and 318 women underwent baseline assessment of health and detailed anthropometric measurements. Muscle strength was measured by grip strength, and physical performance was determined by gait speed. Peripheral quantitative computed tomography (pQCT) examination of the calf and forearm was performed to assess muscle cross-sectional area (mCSA) at the 66% level and bone structure (radius 4% and 66% levels; tibia 4% and 38% levels). Muscle size was positively associated with bone size (distal radius total bone area β = 17.5 mm2/SD [12.0, 22.9]) and strength (strength strain index (β = 23.3 mm3/SD [18.2, 28.4]) amongst women (p < 0.001). These associations were also seen in men and were maintained after adjustment for age, height, weight-adjusted-for-height, limb-length-adjusted-for-height, social class, smoking status, alcohol consumption, calcium intake, physical activity, diabetes mellitus, and in women, years since menopause and estrogen replacement therapy. Although grip strength showed similar associations with bone size and strength in both sexes, these were substantially attenuated after similar adjustment. Consistent relationships between gait speed and bone structure were not seen. We conclude that although muscle size and grip strength are associated with bone size and strength, relationships between gait speed and bone structure and strength were not apparent in this cohort, supporting a role for the muscle-bone unit. © 2013 American Society for Bone and Mineral Research
Osteoporotic fractures are associated with considerable morbidity, mortality, and economic costs.[1, 2] The propensity to fracture is dependent upon the mechanical strength of a bone balanced against the forces it must endure. Sarcopenia, the age-related loss of muscle mass and function, may add to fracture risk by increasing falls risk. However, the mechanostat hypothesis suggests that bones adapt to mechanical loads generated by voluntary mechanical usage, including that of muscle contraction, implying the direct role of muscle on bone structure and strength. Genetic, developmental, endocrine, and lifestyle factors, such as physical activity, have dual effects on both muscle and bone.[6-9] Although others have attempted to examine these relationships, previous studies have focused on associations between muscle measurements with bone mass and density[10-12] but not bone structure. Because mechanical loading preferentially increases bone size above mass and density, neglecting the assessment of bone structure in the association between sarcopenia and fracture risk may lead to an incomplete understanding. Furthermore, studies examining relationships between physical performance and bone structure are limited, and those investigating the effects of muscle size or strength often fail to adequately consider the role of potential confounding factors such as body size, diet, and lifestyle.[14-17] The Hertfordshire Cohort Study is well placed to examine this issue because of the wealth of phenotypic data available relating to body build and musculoskeletal health.
We aimed to study associations between the three muscle components of sarcopenia (low muscle mass; low muscle function defined as low muscle strength; low physical performance) and measures of bone strength, as well as cortical and trabecular bone structure using peripheral quantitative computed tomography (pQCT).
Materials and Methods
The Hertfordshire Cohort Study (HCS) is a population-based cohort study in the United Kingdom that was designed to examine the relationship between growth in infancy and the subsequent risk of adult disease, including osteoporosis. Study design and recruitment have been described in detail previously. Briefly, in conjunction with the National Health Service Central Registry and the Hertfordshire Family Health Service Association, we traced men and women who were born between 1931 and 1939 in Hertfordshire and still lived there during the period 1998 to 2003. In 2004 to 2005, 437 men and 447 women from the geographical area of East Hertfordshire were invited for a follow-up study. Of these, 322 men (65%) and 320 women (68%) agreed to participate.
Interviews and examination
A detailed questionnaire was administered to obtain information on lifestyle, medical history, cigarette smoking, and alcohol consumption. Details regarding physical activity, dietary calcium intake, socioeconomic status, and, in women, years since menopause and use of estrogen replacement therapy had already been obtained from a questionnaire that was administered by trained nurses when the participants were initially recruited into the HCS (1998 to 2003). Physical activity was calculated as a standardized score ranging from 0 to 100 derived from frequency of gardening, housework, climbing stairs, and carrying loads in a typical week. Higher scores indicated greater levels of activity. Dietary calcium intake was assessed using a food-frequency questionnaire. Socioeconomic status was determined using own current or most recent occupation of the participant in men and single women and of the husband in ever-married women based on the OPCS Standard Occupational Classification scheme for occupation and social class.
Height was measured to the nearest 0.1 cm using a Harpenden pocket stadiometer (Chasmors Ltd, London, UK) and weight to the nearest 0.1 kg on a SECA floor scale (Chasmors Ltd). Body mass index (BMI) was calculated as weight divided by height2 (kg/m2). Grip strength was measured three times in each hand using a Jamar (Loughborough, United Kingdom) hand-held isokinetic dynamometer using a standardized protocol. The maximum value was used in analyses. Gait speed was quantified from the time taken to complete a 3-meter walk test.
Peripheral quantitative computed tomography (pQCT)
Nine men and two women either declined or were unable to undergo pQCT. In total, 313 men and 318 women underwent scanning at the radius and tibia (nondominant side) using a Stratec (Pforzheim, Germany) 2000XL pQCT scanner running software version 6.00. The radial length was measured from the distal end of the ulna styloid to the tip of the olecranon in millimeters (mm). The tibial length was measured from the prominence of the medial malleolus to the tibial plate (mm). Forearm and lower leg scout views identified measurement reference lines at the cortical end plates. Two slices were taken in the forearm scan: 4% distal radius and 66% radial midshaft and forearm cross-sectional area (CSA) of muscle. Three slices were taken for the lower leg scan: 4% distal tibia, 38% tibial midshaft, and 66% calf CSA. Assessment of muscle size by pQCT has been found to be valid and reliable. Trabecular parameters were measured distally, and cortical parameters were measured in the midshaft (radius, 66%; tibia, 38%). Measurements were taken from both the radius and tibia of distal total bone area (dTBA), trabecular bone mineral density (tBMD), cortical bone mineral density (cBMD), cortical bone area (cBA), and polar strength strain index (SSI). Midshaft periosteal circumference (PC) and midshaft endosteal circumference (EC) where then calculated assuming that the bone had a circular cross section. Measurement precision error, expressed as a coefficient of variation, ranged from 0.88% (tibial total density, 4% slice) to 8.8% (total radial area, 66% slice) but was typically around 1% to 3%. These figures were obtained by 20 volunteers who were part of the study undergoing two scans on the same day, with limb repositioning between examinations.
For all scans, a threshold of 280 mg/cm3 was used to separate the bone from the soft tissue background. Once separated, the default peeling algorithm was applied to the distal 4% scans to separate trabecular bone. With this peeling, 55% of the outer bone area was concentrically separated and defined as cortical and subcortical; the remaining 45% was defined as trabecular bone. For proximal scan locations, the default threshold of 710 mg/cm3 was used to separate cortical bone. Muscle CSA at the forearm and calf was derived using the default analysis steps that utilize various threshold and edge tracking settings to segment muscle from subcutaneous fat. We chose to adopt this algorithm because it allowed better comparability for our work with other studies; as we hope to perform follow-up scans on this group, it will allow consistency in our approach. Those with significant movement artefact were excluded from analyses.
Ethical approval was granted from the Hertfordshire Research Ethics Committee, and all participants gave written informed consent in accordance with the Declaration of Helsinki.
Study participant characteristics for continuous variables were calculated as means (standard deviation [SD]). Categorical and binary variables were summarized as numbers and percentages of the total study population. All data were visually inspected for normality and loge transformed as appropriate. Measures of sarcopenia for each individual were then converted to a standardized score that represented the number of standard deviations the value was from the mean of the study population. Primary analysis used linear regression to examine the associations between each of these standardized measures of sarcopenia: i) muscle size (forearm and calf muscle CSA); ii) muscle strength (grip strength); and iii) physical performance (gait speed), and pQCT bone parameters in the corresponding limb. For forearm muscle size and grip strength, the corresponding limb was the radius, and for calf muscle size and gait speed it was the tibia. This analysis was repeated with and without adjustment for a priori confounders: age, height, weight-adjusted-for-height, limb-length-adjusted-for-height, social class, smoking status, alcohol consumption, calcium intake, physical activity, diabetes mellitus, and in women, years since menopause and estrogen replacement therapy. EC was additionally adjusted for PC to take into consideration bone size.
Because skeletal size is a key determinant of muscle and bone parameters, consideration was taken of height, weight, and limb length. To avoid overadjustment, the latter two were adjusted for height before inclusion in the model. The model also included adjustment for diabetes (DM), which is known to influence both muscle and bone parameters.[24-27] Bonferroni correction was retrospectively applied to each association to take account of multiple testing.
The mean age of men and women in the study was 69.2 and 69.5 years, respectively (Table 1). All women were postmenopausal. Men were taller, heavier, and had higher calcium intakes. Among men, 61.7% were current or ex-smokers compared with only 36.8% of women. Men were more likely to be heavy alcohol drinkers. Approximately 1 in 5 participants had fractured since the age of 45 years. Two-fifths of men and three-fifths of women reported falls since the age of 45 years. DM had been diagnosed in 15.7% of men and in 12.7% of women. Men had bigger muscles in both their upper and lower limbs, stronger grip strength, and walked faster than women (Table 2). As expected in both the radius and tibia, measures of bone size and density were higher in men than in women.
|Total no.||Men||Total no.||Women||p Value|
|Mean (SD)||Mean (SD)|
|Age (years)||313||69.2 (2.5)||318||69.5 (2.6)||0.111|
|Height (cm)||313||173.7 (6.5)||318||160.5 (6.1)||<0.001|
|Weight (kg)||313||82.0 (11.9)||318||71.8 (13.8)||<0.001|
|Body mass index (kg/m2)a||313||26.9 (1.14)||318||27.4 (1.19)||0.144|
|Radius length (mm)||304||263.3 (11.9)||316||239.1 (13.2)||<0.001|
|Tibia length (mm)||295||379.0 (21.5)||298||348.1 (19.9)||<0.001|
|Physical activity score||313||64.0 (14.2)||318||61.8 (14.3)||0.059|
|Calcium intake (mg/day)a||313||1208.4 (1.29)||318||1094.2 (1.30)||<0.001|
|n (%)||n (%)|
|≥1 fall since age 45 yearsb||312||125 (40.1)||317||194 (61.2)||<0.001|
|Fracture since age 45 yearsc||305||56 (18.4)||316||68 (21.5)||0.325|
|Vitamin D status (nmol/L)|
|<25||259||30 (11.6)||292||49 (16.8)||0.047|
|25–50||123 (47.5)||150 (51.4)|
|>50||106 (40.9)||93 (31.8)|
|Non-drinker||313||9 (2.8)||318||54 (17.0)||<0.001|
|≤recommended||234 (74.8)||256 (80.5)|
|>recommended||70 (22.4)||8 (2.5)|
|Current||313||26 (8.3)||315||17 (5.4)||<0.001|
|Ex||167 (53.4)||99 (31.4)|
|Never||120 (38.3)||199 (63.2)|
|I–IIINM||297||128 (43.1)||318||136 (42.8)||0.934|
|IIIM–V||169 (56.9)||182 (57.2)|
|>5 years ago||58 (18.2)|
|<5 years ago||20 (6.3)|
|Years since menopause|
|Total no.||Men (n = 304)||Total no.||Women (n = 316)||p Value|
|Mean (SD)||Mean (SD)|
|Forearm muscle CSA (mm2)||304||4033.2 (517.6)||314||2554.9 (369.7)||<0.001|
|Calf muscle CSA (mm2)||293||8035.2 (1203.6)||295||6212.2 (980.9)||<0.001|
|Grip strength (kg)||312||42.2 (7.6)||317||24.9 (5.8)||<0.001|
|Gait speed (m/s)||311||0.92 (0.17)||314||0.88 (0.16)||<0.001|
|dTBA||304||493.8 (68.8)||316||371.3 (51.9)||<0.001|
|tBMD||304||211.6 (40.5)||316||173.1 (44.7)||<0.001|
|cBMD||304||1113.6 (39.7)||316||1095.5 (46.4)||<0.001|
|cBA||304||99.5 (13.7)||316||63.7 (12.1)||<0.001|
|PC||304||47.7 (3.6)||316||40.8 (3.3)||<0.001|
|EC||304||31.9 (4.7)||316||29.3 (4.3)||<0.001|
|SSI||304||397.5 (81.9)||316||226.1 (51.1)||<0.001|
|dTBA||295||1391.3 (163.4)||298||1129.8 (128.2)||<0.001|
|tBMD||295||241.9 (36.5)||298||220.6 (45.6)||<0.001|
|cBMD||295||1155.8 (26.4)||298||1141.1 (37.4)||<0.001|
|cBA||295||338.6 (39.9)||298||247.8 (32.2)||<0.001|
|PC||295||77.5 (4.2)||298||68.8 (3.9)||<0.001|
|EC||295||41.9 (4.8)||298||40.0 (5.4)||<0.001|
|SSI||295||2072.3 (328.2)||298||1400.7 (226.9)||<0.001|
Clear positive associations were found between forearm muscle size and radial bone size (dTBA, cBA, PC) and bone strength (SSI) in men (Fig. 1). However, no association was observed between forearm muscle size and radial bone mineral density (cBMD, tBMD). Similar patterns were found in the lower limb between calf muscle size and tibial bone parameters (Table 3). In women, as in men, muscle size in the forearm and calf was positively associated with both bone size (dTBA, cBA, PC) and bone strength (SSI) (Fig. 2). However, in contrast to men, muscle size was also positively associated with tBMD, but not cBMD, in both upper and lower limbs.
|β (95% CI)||β (95% CI)|
|Forearm muscle CSA (muscle size) and radial bone structure|
|dTBA||25.0 (17.7, 32.2)***||17.5 (12.0, 22.9)***|
|tBMD||2.34 (−2.24, 6.91)||10.1 (5.25, 14.9)***|
|cBMD||−4.25 (−8.72, 0.22)||3.53 (−1.64, 8.70)|
|cBA||5.11 (3.68, 6.55)***||5.91 (4.75, 7.08)***|
|PC||1.40 (1.02, 1.77)***||1.34 (1.02, 1.67)***|
|ECa||−0.63 (−0.90, −0.35)***||−1.05 (−1.32, −0.78)***|
|SSI||27.2 (18.4, 35.9)***||23.3 (18.2, 28.4)***|
|Calf muscle CSA (muscle size) and tibial bone structure|
|dTBA||40.6 (22.4, 58.8)***||37.3 (23.3, 51.4)***|
|tBMD||3.84 (−0.35, 8.03)||10.2 (5.06, 15.3)***|
|cBMD||−2.42 (−5.45, 0.62)||−0.71 (5.02, 3.60)|
|cBA||14.1 (9.88, 18.4)***||15.7 (12.5, 19.0)***|
|PC||1.58 (1.34, 2.02)***||1.78 (1.39, 2.18)***|
|ECa||−0.45 (−0.91, −0.01)*||−1.38 (−1.86, −0.90)***|
|SSI||115 (80.0, 150)***||112 (88.9, 134)***|
|Grip strength (muscle strength) and radial bone structure|
|dTBA||12.4 (4.70, 20.1)**||9.52 (3.87, 15.2)**|
|tBMD||−1.26 (−5.85, 3.34)||0.35 (−4.61, 5.32)|
|cBMD||0.73 (−3.76, 5.23)||5.45 (0.34, 10.56)*|
|cBA||2.15 (0.62, 3.69)**||1.78 (0.45, 3.11)**|
|PC||0.55 (0.15, 0.96)**||0.18 (−0.18, 0.54)|
|ECa||−0.24 (−0.50, 0.20)||−0.31 (−057, −0.04)*|
|SSI||12.3 (3.13, 21.5)**||8.76 (3.16, 14.4)**|
|Gait speed (physical performance) and tibial bone structure|
|dTBA||15.5 (−3.32, 34.2)||−5.05 (−20.0, 9.87)|
|tBMD||−1.81 (−6.04, 2.42)||3.40 (−1.94, 8.75)|
|cBMD||0.57 (−2.48, 3.61)||−0.67 (−5.07, 3.73)|
|cBA||4.15 (−0.45, 8.76)||1.80 (−1.97, 5.58)|
|PC||0.43 (−0.04, 0.91)||−0.42 (−0.88, 0.03)|
|ECa||−0.14 (−0.57, 0.30)||−0.56 (−1.02, −0.11)*|
|SSI||42.7 (5.03, 80.3)*||−9.96 (−36.5, 16.6)|
In both sexes, after adjustment for age, height, weight-adjusted-for-height, limb-length-adjusted-for-height, social class, smoking status, alcohol consumption, calcium intake, physical activity, DM, and in women, years since menopause and estrogen replacement therapy, positive associations between muscle size and both bone size (dTBA, cBA, PC) and bone strength (SSI) in the corresponding limb all persisted (Table 4).
|β (95% CI)||β (95% CI)|
|Forearm Muscle CSA (muscle size) and radial bone structure|
|dTBA||23.9 (14.6, 33.3)***||16.8 (10.4, 23.1)***|
|tBMD||2.63 (−3.33, 8.60)||8.09 (2.30, 13.9)**|
|cBMD||−2.38 (−8.08, 3.32)||−0.24 (−6.50, 6.01)|
|cBA||4.28 (2.36, 6.20)***||5.25 (3.84, 6.66)***|
|PC||1.20 (0.71, 1.69)***||1.44 (1.05, 1.83)***|
|ECa||−0.48 (−0.83, −0.13)**||−0.90 (−1.23, −0.58)***|
|SSI||23.6 (12.2, 35.1)***||24.6 (18.6, 30.6)***|
|Calf muscle CSA (muscle size) and tibial bone structure|
|dTBA||22.8 (1.78, 43.9)*||19.5 (4.09, 34.9)*|
|tBMD||−0.35 (−5.61, 4.91)||3.65 (−2.60, 9.90)|
|cBMD||−1.39 (−5.42, 2.64)||−4.13 (−9.53, 1.27)|
|cBA||8.57 (3.20, 14.0)**||9.71 (5.97, 13.4)***|
|PC||1.18 (0.65, 1.72)***||1.32 (0.87, 1.77)***|
|ECa||−0.04 (−0.61, 0.54)||−0.86 (−1.41, −0.32)**|
|SSI||78.2 (35.7, 121)***||76.4 (51.3, 102)***|
|Grip strength (muscle strength) and radial bone structure|
|dTBA||9.66 (0.84, 18.5)*||5.97 (0.19, 11.7)*|
|tBMD||−1.94 (−7.38, 3.50)||−0.30 (−5.46, 4.86)|
|cBMD||−0.68 (−5.86, 4.51)||2.80 (−2.64, 8.23)|
|cBA||1.43 (−0.38, 3.23)||0.78 (−0.57, 2.12)|
|PC||0.50 (0.04, 0.96)*||−0.04 (−0.41, 0.34)|
|ECa||−0.11 (−0.42, 0.21)||−0.15 (−0.43, 0.12)|
|SSI||8.59 (−2.10, 19.3)||4.17 (−1.64, 9.98)|
|Gait speed (physical performance) and tibial bone structure|
|dTBA||2.20 (−15.9, 20.3)||−3.63 (−16.9, 9.68)|
|tBMD||−0.21 (−4.65, 4.23)||3.88 (−1.41, 9.17)|
|cBMD||−1.30 (−4.69, 2.09)||−1.04 (−5.64, 3.56)|
|cBA||3.99 (−0.66, 8.64)||3.20 (−0.12, 6.51)|
|PC||0.40 (−0.07, 0.87)||−0.22 (−0.63, 0.19)|
|ECa||−0.15 (−0.62, 0.32)||−0.57 (−1.02, −0.13)*|
|SSI||37.1 (0.63, 73.5)*||0.96 (−22.0, 23.9)|
We considered relationships between muscle size and the cortical and trabecular compartments. In general, EC was inversely related to muscle size; this was most apparent in women (Table 4). Overall, after adjustment for confounders, muscle size was independent of both cortical and trabecular BMD, with the exception of a persistent positive relationship between forearm muscle size and radial tBMD in women. After correction for multiple testing, the majority of associations between muscle size and bone structure were maintained, despite partial attenuation (data not shown).
Grip strength (muscle strength)
Positive relationships were identified between grip strength and both radial bone size (dTBA, cBA, PC) and bone strength (SSI) in men (Fig. 1). Similar relationships were seen in women, although associations with bone size were weaker (Fig. 2). Although adjustment fully attenuated our observed associations between grip strength and bone strength, the associations with bone size persisted in both men and women (Table 4). In general, BMD was independent of grip strength.
Gait speed (physical performance)
In contrast to muscle size and grip strength, analyses found no evidence to support an association between gait speed and any of the tibial bone parameters in men (Fig. 1) or women (Fig. 2) that were robust to adjustment for multiple testing and confounders.
We have shown that muscle size is strongly associated with bone size and bone strength in both men and women and that these relationships remain robust after rigorous adjustment. By contrast, gait speed was not associated with measures of bone size, strength, or density in this cohort of older adults. These data support the mechanostat hypothesis with associations between both muscle size and grip strength with bone size likely to arise through dynamic loading.
The associations of muscle size with both bone size and strength, that we have shown, are in keeping with published findings.[16, 17, 29-31] However, in contrast to most previous studies, we were able to look not only at cBA but also specifically at changes in periosteal and endosteal circumferences. In both weight-bearing and non-weight-bearing limbs, we found greater muscle size was invariably associated with larger PC and bone size. Conversely, EC tended to decrease after adjustment for PC, in particular in women. This indicates that the greater cBA is likely to reflect contributions from increased PC and reduced EC. The greater effect in women than in men might suggest a role for estrogen in this relationship.
The positive associations between tBMD and muscle size in weight-bearing and non-weight-bearing limbs were attenuated after adjustment. This observation has previously only been made in children. We found no evidence to support an association between muscle size and cBMD, which is consistent with animal studies that show cortical bone adapts its strength to mechanical loading by preferentially increasing bone size rather than BMD.
Positive associations were found between grip strength and both bone strength (SSI) and size (dTBA, cBA, and PC) in men and women, consistent with what has been found previously.[15, 32] However, we were uniquely positioned to take account of potential covariates,[14, 17] and this may explain why we observed a greater attenuation after adjustment than had been previously demonstrated. Furthermore, the published literature describing grip strength and BMD relationships are heterogeneous in terms of ethnicity, exclusion criteria, assessment of confounders, study size, and conclusions, and fail to reach a consensus.[14, 15, 33]
To our knowledge, the relationship between physical performance as measured by gait speed and bone structure has not been previously investigated. The STRAMBO study defined physical performance in terms of chair rises and static and dynamic balance, and found that those participants who failed on more than one of these tests had significantly lower BMD (trabecular, cortical, and total) and cortical bone area as assessed by high-resolution pQCT. Although our definition of PP is different, it is also possible that our cohort was generally fitter than the participants from the STRAMBO study.
Associations between muscle and bone size could be explained in several ways. First, the mechanostat hypothesis states that a muscle provides a direct mechanical stimulus to a bone during contraction to generate bending moments. Mechanotransduction of these forces subsequently occurs mainly through the function of osteocytes, which sense strain and regulate sclerostin production in response. This allows deforming forces applied to bone to promote osteogenesis. This hypothesis forms a possible mechanism through which muscle and bone may be linked. Second, genetic and hormonal factors have pleiotropic effects on the musculoskeletal system and can, either directly or indirectly, impact on both muscle and bone growth.[6-8, 36, 37] Third, exercise can affect both bone structure and muscle size and strength.[38-40] Finally, in keeping with the developmental origins hypothesis, there is evidence supporting associations between birth weight and both bone health and muscle function in later life. Consequently, a common developmental contribution to both facets could partly explain associations found.
The main strengths of our study are that the sample investigated is generally representative of the UK population as a whole and that previous extensive phenotyping has allowed us to thoroughly adjust for potential confounding factors. However, there are also several limitations of this study. First, the design was cross-sectional, which prevents assessment of causality. Grip strength was taken as the highest of six measurements (three on the dominant side and three on the nondominant side) and so usually the maximum grip strength measurement was taken from the dominant side, whereas bone parameters were assessed on the nondominant side. This may attenuate associations found between grip strength and bone parameters to a degree, although the difference between dominant and nondominant limbs is usually small and relatively consistent. Second, the pQCT assessment of bone strength provided is a theoretical estimate, rather than a measure of experimental failure. However, it has previously been shown to be related to in vitro breaking strength. Third, it is clear that the radius and tibia are not completely circular in cross section. The manufacturer of the instrument recommends use of a circular ring model assumption in analysis of pQCT images; furthermore, images analyzed on this basis have been shown to be accurate at both skeletal sites.[46, 47] A potential alternative strategy would be to use an iterative contour detection method, which might provide a direct measurement based on the true shape of the bone. However, this procedure fails to produce a measurement that can be utilized in an appreciable portion of individuals. As a consequence, our main data were presented using a circular ring model assumption as suggested by the manufacturer. Finally, we also recognize the limitations associated with self-reported physical activity, dietary calcium, smoking status, and alcohol consumption.
In conclusion, we have shown that muscle size and grip strength, but not gait speed, are associated with bone size and strength. Potential mechanisms explaining this relationship include the mechanostat hypothesis and developmental, genetic, and hormonal factors. Further studies are needed to determine whether muscle size and grip strength have a deterministic relationship with bone structure and whether associations also exist between each of the muscle parameters and bone-related clinical outcomes such as fractures.
CC has received honoraria and consulting fees from Amgen, Eli Lilly, Medtronic, Merck, Novartis, and Servier. All other authors state that they have no conflicts of interest.
The Hertfordshire Cohort Study was supported by the Medical Research Council of Great Britain; Arthritis Research UK; and the International Osteoporosis Foundation. The work herein was also supported by the NIHR Nutrition BRC, University of Southampton and the NIHR Musculoskeletal BRU, University of Oxford. MHE is funded by an Arthritis Research UK Clinical PhD Studentship. We thank all of the men and women who took part in the Hertfordshire Cohort Study; the HCS Research Staff; and Vanessa Cox, who managed the data.
Authors' roles: MHE, EMD, and CC made substantial contributions to the conception and design of the study. EMD and CC were involved in data acquisition. MHE drafted the manuscript with input from CLG, ED, and CC. All authors contributed to the analysis and interpretation of the data, revised the manuscript critically for important intellectual content, and approved the final version of the submitted manuscript. MHE accepts responsibility for the integrity of the data analysis.