Additional Supporting Information may be found in the online version of this article.


Fig. S1. Deletion of Bak and Bax does not affect femoral size, growth plate morphology, or cortical thickness. (A) Length and (B) Safranin-O-stained histological sections of the distal femur from 3-month-old mice. Scale bar, 200 µm. (C) Cortical femoral thickness measured at the mid-diaphysis by micro-CT. Femurs are from the same mice shown in Figures 2 and 3.

Fig. S2. Deletion of Bak and Bax with OCN-Cre or Osx1-Cre has no effect on vertebral cancellous bone volume. Vertebral cancellous bone volume determined by micro-CT of (A) L4 from BakΔBaxf/f mice and BakΔBaxΔOCN mice of the experiment described in Figure 2; and (B) L4 from BakΔOsx1-Cre and BakΔBaxΔOsx1 mice of the experiment described in Figure 3.

Fig. S3. Increased mineral deposition and expression of alkaline phosphatase in ex-vivo cultures of marrow-derived osteoblast progenitors from BakΔBaxΔOCN mice. Ex-vivo marrow cultures were established from 2-3 month old female and male mice, and maintained in αMEM supplemented with 10% FBS and 1 mM ascorbate-2-phosphate for 20 days. (A) Von Kossa staining to detect mineral deposition. (B) Alkaline phosphatase (Akp2) and osteocalcin (Ocn) mRNA level, relative to ChoB. n = 3/group. *P< 0.05.

Fig. S4. Deletion of Bak and Bax does not increase cortical porosity in young mice. Representative micro-CT images from female mice at 3 months of age, or male mice at 8 months of age, from the experiment shown in Figure 2. Scale bar, 1mm.

jbmr2007-sm-0002-sm-SuppVideo.avi1811KSupplementary Video

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