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Additional Supporting Information may be found in the online version of this article.

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jbmr2007-sm-0001-sm_SuppFigs-S1-4.ppt1045K

Fig. S1. Deletion of Bak and Bax does not affect femoral size, growth plate morphology, or cortical thickness. (A) Length and (B) Safranin-O-stained histological sections of the distal femur from 3-month-old mice. Scale bar, 200 µm. (C) Cortical femoral thickness measured at the mid-diaphysis by micro-CT. Femurs are from the same mice shown in Figures 2 and 3.

Fig. S2. Deletion of Bak and Bax with OCN-Cre or Osx1-Cre has no effect on vertebral cancellous bone volume. Vertebral cancellous bone volume determined by micro-CT of (A) L4 from BakΔBaxf/f mice and BakΔBaxΔOCN mice of the experiment described in Figure 2; and (B) L4 from BakΔOsx1-Cre and BakΔBaxΔOsx1 mice of the experiment described in Figure 3.

Fig. S3. Increased mineral deposition and expression of alkaline phosphatase in ex-vivo cultures of marrow-derived osteoblast progenitors from BakΔBaxΔOCN mice. Ex-vivo marrow cultures were established from 2-3 month old female and male mice, and maintained in αMEM supplemented with 10% FBS and 1 mM ascorbate-2-phosphate for 20 days. (A) Von Kossa staining to detect mineral deposition. (B) Alkaline phosphatase (Akp2) and osteocalcin (Ocn) mRNA level, relative to ChoB. n = 3/group. *P< 0.05.

Fig. S4. Deletion of Bak and Bax does not increase cortical porosity in young mice. Representative micro-CT images from female mice at 3 months of age, or male mice at 8 months of age, from the experiment shown in Figure 2. Scale bar, 1mm.

jbmr2007-sm-0002-sm-SuppVideo.avi1811KSupplementary Video

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