Dysapoptosis of Osteoblasts and Osteocytes Increases Cancellous Bone Formation But Exaggerates Cortical Porosity With Age
Version of Record online: 19 DEC 2013
© 2014 American Society for Bone and Mineral Research
Journal of Bone and Mineral Research
Volume 29, Issue 1, pages 103–117, January 2014
How to Cite
Jilka, R. L., O'Brien, C. A., Roberson, P. K., Bonewald, L. F., Weinstein, R. S. and Manolagas, S. C. (2014), Dysapoptosis of Osteoblasts and Osteocytes Increases Cancellous Bone Formation But Exaggerates Cortical Porosity With Age. J Bone Miner Res, 29: 103–117. doi: 10.1002/jbmr.2007
- Issue online: 19 DEC 2013
- Version of Record online: 19 DEC 2013
- Accepted manuscript online: 12 JUN 2013 07:45AM EST
- Manuscript Accepted: 3 JUN 2013
- Manuscript Revised: 22 MAY 2013
- Manuscript Received: 3 DEC 2012
- U.S. Department of Veterans Affairs, the Biomedical Laboratory Research and Development Service of the VA Office of Research and Development (Research Career Scientist award and. Grant Number: I01 BX000514 to RLJ; I01 BX000436 to RSW; I01 BX000294 to CAO; and I01 BX001405 to SCM
- National Institutes of Health. Grant Number: P01 AG013918 to SCM; and P01 AR46798 to LFB
- UAMS Translational Research Institute. Grant Number: 1UL1RR029884
Additional Supporting Information may be found in the online version of this article.
Fig. S1. Deletion of Bak and Bax does not affect femoral size, growth plate morphology, or cortical thickness. (A) Length and (B) Safranin-O-stained histological sections of the distal femur from 3-month-old mice. Scale bar, 200 µm. (C) Cortical femoral thickness measured at the mid-diaphysis by micro-CT. Femurs are from the same mice shown in Figures 2 and 3.
Fig. S2. Deletion of Bak and Bax with OCN-Cre or Osx1-Cre has no effect on vertebral cancellous bone volume. Vertebral cancellous bone volume determined by micro-CT of (A) L4 from BakΔBaxf/f mice and BakΔBaxΔOCN mice of the experiment described in Figure 2; and (B) L4 from BakΔOsx1-Cre and BakΔBaxΔOsx1 mice of the experiment described in Figure 3.
Fig. S3. Increased mineral deposition and expression of alkaline phosphatase in ex-vivo cultures of marrow-derived osteoblast progenitors from BakΔBaxΔOCN mice. Ex-vivo marrow cultures were established from 2-3 month old female and male mice, and maintained in αMEM supplemented with 10% FBS and 1 mM ascorbate-2-phosphate for 20 days. (A) Von Kossa staining to detect mineral deposition. (B) Alkaline phosphatase (Akp2) and osteocalcin (Ocn) mRNA level, relative to ChoB. n = 3/group. *P < 0.05.
Fig. S4. Deletion of Bak and Bax does not increase cortical porosity in young mice. Representative micro-CT images from female mice at 3 months of age, or male mice at 8 months of age, from the experiment shown in Figure 2. Scale bar, 1mm.
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