Paget's disease of bone (PDB) is characterized by abnormal osteoclasts with unique characteristics that include increased sensitivity of osteoclast progenitors to 1,25(OH)2D3, receptor activator of NF-κB ligand (RANKL), and TNF-α; increased osteoclast numbers; and increased expression of IL-6 and several transcription factors. We recently reported that measles virus nucleocapsid protein (MVNP) plays a key role in the development of these abnormal osteoclasts. MVNP can induce the pagetic osteoclast phenotype in vitro and in vivo in TRAP-MVNP transgenic mice. However, the molecular mechanisms by which MVNP generates pagetic osteoclasts have not been determined. TANK-binding kinase 1 (TBK1) and IκB kinase-ϵ (IKKϵ) are IKK family members that complex with MVNP and activate both IRF3 and NF-κB pathways. MVNP increases the amount of TBK1 protein in bone marrow monocytes (BMM). Interestingly, we found that RANKL increased TBK1 and IKKϵ early in osteoclast differentiation, suggesting a possible role in normal osteoclastogenesis. However, only TBK1 is further increased in osteoclasts formed by TRAP-MVNP BMM owing to increased TBK1 protein stability. TBK1 overexpression induced IL6 promoter reporter activity, and elevated endogenous IL6 mRNA and p65 NF-κB, TAF12, and ATF7 proteins in several cell lines. Overexpression of TBK1 was insufficient to induce pagetic osteoclasts from WT BMM but synergized with MVNP to increase pagetic osteoclast formation from TRAP-MVNP BMM. BX795 inhibition of TBK1 impaired MVNP-induced IL-6 expression in both NIH3T3 cells and BMM, and shRNA knockdown of Tbk1 in NIH3T3 cells impaired IL-6 secretion induced by MVNP and decreased TAF12 and ATF7, factors involved in 1,25(OH)2D3 hypersensitivity of pagetic osteoclasts. Similarly, Tbk1 knockdown in BMM from TRAP-MVNP and WT mice specifically impaired development of the MVNP-induced osteoclast pagetic phenotype. These results demonstrate that TBK1 plays a critical role in mediating the effects of MVNP on osteoclast differentiation and on the expression of IL-6, a key contributor to the pagetic osteoclast phenotype. © 2014 American Society for Bone and Mineral Research.
Paget's disease of bone is characterized by abnormal osteoclasts that express a “pagetic phenotype” that distinguishes them from normal osteoclasts, which includes increased sensitivity of osteoclast progenitors to 1,25(OH)2D3, receptor activator of NF-κB ligand (RANKL), and TNF-α; increased rate of formation and numbers of osteoclasts and nuclei/osteoclast; enhanced bone resorption capacity; and increased expression of the transcription regulator TAF12 and the cytokine IL-6.[1-3] Both environmental factors, such as measles virus, and genetic alterations have been implicated in the pathogenesis of Paget's disease of bone.[4, 5] The only gene in which protein-coding mutations are causally linked to Paget's disease of bone is sequestosome 1, which encodes p62, a scaffold protein that is involved in signaling by several cytokines, including RANKL. The most common mutation is p62P392L, an autosomal dominant mutation with variable penetrance. However, mutant p62 alone does not appear to be capable of inducing Paget's disease of bone because we found that mice with the murine equivalent of p62P392L (p62P394L) knocked-in or overexpressing p62P392L have increased osteoclast numbers, but the osteoclasts do not have a pagetic phenotype, nor do the mice develop the bone lesions characteristic of Paget's disease.[1, 7] Further, transfection of p62P392L cDNA into normal osteoclast precursors does not result in formation of pagetic-like osteoclasts in vitro. Previously, we reported that 70% to 80% of Paget's disease patients expressed measles virus nucleocapsid protein (MVNP),[1, 9] and transduction of normal osteoclast precursors with MVNP cDNA results in development of osteoclasts that express the characteristics of pagetic osteoclasts. In addition, we also demonstrated that MVNP can induce the pagetic osteoclast phenotype in vitro and in vivo in TRAP-MVNP transgenic mice, in which the TRAP promoter drives MVNP expression in osteoclast lineage cells. Most importantly, we recently showed that antisense knockdown of MVNP expression in MVNP-positive osteoclasts from Paget's patients harboring a p62 mutation resulted in loss of the pagetic phenotype.[1, 9] Further, when mice carrying the mutant p62 knock-in were bred to TRAP-MVNP mice, the p62/MVNP mice developed greater numbers of pagetic osteoclasts and dramatic pagetic bone lesions that were strikingly similar to those seen in Paget's disease.[1, 9] However, the molecular mechanisms responsible for the capacity for MVNP to induce osteoclasts that express a pagetic phenotype are not well understood.
MVNP activates the antiviral response pathway by interacting with a complex containing the IκB kinase (IKK)-related homologous kinases, TANK-binding kinase 1 (TBK1) and IKKϵ (gene name IKBKE), and the transcription factor interferon regulatory factor 3 (IRF3).[12, 13] MVNP binding results in activation of TBK1 and IKKϵ, which in turn phosphorylate the IRF3 C-terminus, thereby activating IRF3 and resulting in nuclear translocation and transcriptional activation of its target genes, such as interferon β (IFNβ).[12, 13] Gene expression profiling studies indicated that IFNβ and associated molecules are upregulated in pagetic peripheral blood monocytes. TBK1 and IKKϵ can also activate NF-κB both indirectly and directly. They interact with the TRAF-binding protein TANK (also called I-TRAF) complexed with TRAF2, and function upstream of IKKα and IKKβ to activate NF-κB indirectly.[15, 16] TBK1 and IKKϵ also can directly phosphorylate p65 NF-κB at Ser468 and Ser536,[17-21] which allows IκB-independent nuclear translocation and potentiates transactivation of transcription.
We previously reported that MVNP increases p65 NF-κB, phospho-Ser536-p65 NF-κB, nuclear NF-κB, and the activation of NF-κB-responsive reporters, including the IL6 gene.[10, 23] In addition, the high levels of IL-6 observed in Paget's disease of bone[2, 24] are induced in osteoclasts by MVNP but not by mutant p62.[1, 7, 8] We have shown that loss of IL-6 expression in TRAP-MVNP mice abrogated the formation of pagetic osteoclasts and bone lesions in vivo.[1, 7, 8] Therefore, the mechanism by which MVNP elevates IL-6 is an important part of the generation of Paget's disease. We hypothesized that MVNP activation of TBK1 and/or IKKϵ contributes to the formation of pagetic osteoclasts either by upstream regulation of IKK-dependent NF-κB activation or by increasing the IKK-independent activity of p65 NF-κB via direct phosphorylation, resulting in increased IL-6 expression. Therefore, we investigated whether TBK1 and/or IKKϵ mediates the effects of MVNP in pagetic osteoclasts, with a particular focus on MVNP upregulation of IL-6 expression.
Materials and Methods
Cell culture media, penicillin, and streptomycin (pen/strep) were from GIBCO-BRL (Grand Island, NY, USA). Fetal calf serum (FCS), protease inhibitor cocktail, and cycloheximide (CHX) were from Sigma-Aldrich (St. Louis, MO, USA). Recombinant murine macrophage colony-stimulating factor (M-CSF), recombinant murine RANKL, recombinant murine IL-6, and quantikine mouse IL-6 ELISA kits were purchased from R&D Systems (Minneapolis, MN, USA). Antibodies were from the following vendors: anti-TBK1 (#3013), anti-phospho-Ser172-TBK1 (#5483), anti-p65 NF-κB (#3034), anti-phospho-Ser536-p65 NF-κB (#3033), and anti-GFP (#2956) from Cell Signaling Technology (Beverly, MA, USA); anti-IKKϵ (HPA015788) and mouse monoclonal antibody against β-actin (A5316) from Sigma-Aldrich; anti-TAF12 (12353-1-AP) was from Protein Tech (Chicago, IL, USA); and anti-ATF7 (ab65064) and anti-MVNP antibody [3E1] (ab9397) were from Abcam (Cambridge, MA, USA). HRP-conjugated IgG secondary antibodies were from GE Healthcare Life Sciences (Little Chalfont, UK). ECL reagents were from GE Healthcare Life Sciences (Buckinghamshire, UK). Polyvinyl difluoride (PVDF) membranes were from Bio-Rad Laboratories (Hercules, CA, USA). The TBK1 inhibitor BX795 was from Invivogen (San Diego, CA, USA).
The Institutional Animal Care and Use Committees at the Virginia Commonwealth University, University of Pittsburgh School of Medicine, and VA Pittsburgh Healthcare System approved all animal studies. Transgenic mice expressing MVNP under the control of the mouse TRAP promoter (TRAP-MVNP mice) were previously described.
Osteoclast formation from mouse bone marrow monocytes (BMM)
Nonadherent BMM were isolated from the tibias of 4- to 6-month-old mice as previously described and cultured at 2 × 105 per well in 96-well plates in 0.1 mL of α-modified Eagle medium (α-MEM) with 10% FCS and M-CSF (50 ng/mL) for 3 days at 37°C in 5% CO2. Osteoclast differentiation was induced by culturing in α-MEM with 10% FCS, M-CSF (50 ng/mL), and RANKL (50 ng/mL), with half of the media changed every other day. After 4 days of osteoclastogenic culture, the cells were fixed with 36.5% formaldehyde for 10 minutes. Osteoclast-like cells were stained for TRAP expression using a leukocyte acid phosphatase kit (Sigma). The TRAP-positive multinucleated cells with ≥3 nuclei were scored. All osteoclast formation experiments were scored from three independent wells.
Cell lines and culture conditions
NIH3T3 cell lines stably transduced with MVNP or empty vector (EV) were previously described. RAW264.7 cell lines stably transduced with MVNP or EV were made using the same vectors and methods described for the NIH3T3 cell lines. Mouse MC3T3-E1 subclone-4 (MC4) pre-osteoblasts were described previously and maintained in ascorbic acid-free α-MEM–10% FCS-1% pen/strep (proliferation media). All the other cell lines, including HEK293, were grown in Dulbecco's modified Eagle medium (DMEM) containing 10% FCS, 1% pen/strep, and 2 mM L-glutamine.
Plasmids and luciferase reporter assay
Constructs expressing wild-type and dominant negative (K38A) human IKKϵ and TBK1 as well as shRNA against human TBK1 were generously received from Dr Tom Maniatis, Harvard University. The pcDNA3.1-MVNP and FLAG-MVNP expression constructs were previously described. Transfection of HEK293, EV/MVNP-NIH3T3, and EV/MVNP-RAW264.7 was done using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). The -1200 IL-6 promoter driven pGL2 reporter was a gift from Dr. Jian Zhang, University of Pittsburgh. All transfections included pRL-CMV as an internal control. Cell lysates were harvested with the Passive Lysis Buffer (Promega, San Luis Obispo, CA, USA), and the luciferase activity of the IL-6 promoter reporter was determined by the dual luciferase assay (Promega) using a GloMax luminometer (Promega).
Lentivirus production and transduction
Mouse Tbk1 shRNA (GCTGGGTGAGATTTCAGACAT) and scrambled shRNA (CCTAAGGTTAAGTCGCCCTCG) lentiviruses (made from vector pLKO.1-puro) were generated by the UPCI lentivirus core facility. For TBK1 overexpression experiments, we inserted the human TBK1 cDNA into the FUGW lentiviral construct (which contains a GFP cDNA) kindly provided by Dr Guoshang Xu, University of Pittsburgh, such that a TBK1-GFP fusion protein was encoded. To produce the lentivirus, supernatants were harvested from 293T cells (6.0 × 106/per 10-cm culture dish) transfected with FUGW or FUGW-TBK1 construct (10 μg), and VSV-G (2.5 μg), RSV-REV (3.5 μg), and PRRE (6.5 μg) constructs in antibiotic-free DMEM via lipofectamine2000 reagent for 48 hours.
NIH3T3 cells were infected with cell-free lentiviral supernatants (106 pfu/mL) in the presence of polybrene (8 μg/mL) as previously described. To transduce mouse primary nonadherent BMM, the lentivirus was first concentrated with lenti-X concentrator (Clontech, Mountain View, CA, USA) according to the manufacturer's instructions. The concentrated lentivirus transduction of BMM was aided by ViroMag R/L based on Magnetofection technology (Oz Biosciences, Marseille, France) carried out according to the manufacturer's instructions. For lentiviral transduction, the nonadherent cells (2 × 105/well) were mixed per well in 100 µL αMEM containing 10% FCS, M-CSF (10 ng/mL), ViroMag R/L beads (2 µL), and 10 µL concentrated lentiviral transduction particles (5 × 107/mL), and incubated for 15 minutes at RT before plating in 96-well culture plates. The plated cell-virus particle-magnet mixtures were incubated on a magnet plate for 60 minutes, and then the cells incubated with or without RANKL at 37°C with 5% CO2 for 4 days.
Enzyme-linked immunosorbent assay (ELISA)
Conditioned media from NIH3T3 cell lines was harvested 38 days after lentivirus infection, and conditioned media from osteoclast cultures was collected 4 days after the addition of RANKL. The concentration of IL-6 present was determined using a Quantikine mouse IL-6 ELISA kit (R&D) according to the manufacturer's instructions.
Protein extraction and Western blot analysis
Collected cells were centrifuged at 500g for 5 minutes, washed with cold phosphate-buffered saline, and lysed in cell lysis buffer (20 mM Tris HCl [pH 7.5], 150 mM NaCl, 1% NP-40, 50 mM NaF, 1 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid, 1 mM ethylenediaminetetraacetic acid, 1 mM β-glycerophosphate, 2.5 mM sodium pyrophosphate, 1 mM orthovanadate, 1× protease inhibitor cocktail, 1 mM phenylmethylsulfonyl fluoride). After centrifugation at 14,000g for 15 minutes at 4°C, equal amounts (10 μg) boiled protein samples were run on a 10% SDS polyacrylamide gel, transferred to PVDF membranes, and probed with primary and the appropriate HRP-conjugated secondary antibodies. Signals were detected using ECL reagents and film. Band densitometric analysis was performed using Image J software (NIH).
RNA extraction and quantitative reverse transcription polymerase chain reaction (qPCR)
Total RNA was isolated from cell pellets using Trizol reagent according to the manufacturer's instructions. Reverse transcription was conducted using a kit (#A3500) from Promega. An aliquot of each cDNA product was used for qPCR analysis to measure the relative mRNA levels using the iQ SYBR Green Supermix and iCycler iQ PCR Detection System (Bio-Rad Laboratories). Samples were normalized to 18S RNA expression. The gene-specific primers used are listed in Table 1.
|Mouse gene name|
|Human gene name|
All the experiments were repeated at least two independent times. Data are presented as the means ± standard deviation. Statistical significance was evaluated by Student's t test with p < 0.05 and p < 0.01 considered to be significant and highly significant, respectively.
MVNP increases total and activated TBK1, in part, by stabilizing TBK1 protein
Because MVNP can activate TBK1 and IKKϵ, we hypothesized that MVNP activation of one or both of these proteins plays a major role in transducing the signals that generate the pagetic phenotype in osteoclasts. We first investigated whether MVNP influenced TBK1 protein levels in RAW264.7 cells (pre-osteoclasts) and NIH3T3 cells. MVNP stably transduced RAW264.7 cells (MVNP-RAW264.7) were generated as previously published for the MVNP-NIH3T3 cells and confirmed by qPCR for MVNP mRNA expression (Supplemental Fig. S1). Western blot analysis indicated that both cell types stably expressing MVNP had increased levels of TBK1 protein compared with cells carrying the empty-vector (EV) (Fig. 1A, left panel). In addition, transient transfection of HEK293 cells with a MVNP expression plasmid dose-dependently increased total TBK1 levels (Fig. 1A, right panel). Importantly, we found BMM isolated from transgenic TRAP-MVNP (TG-MVNP) mice, which selectively express MVNP in osteoclasts and their precursors, had increased TBK1 but not IKKϵ protein levels when compared with BMM from WT mice (Fig. 1B, day 0). Interestingly, RANKL treatment increased both TBK1 and IKKϵ protein levels in WT and TRAP-MVNP mice, with similar kinetics, both peaking at day 1, but again only TBK1 was increased to a greater extent in MVNP-expressing cells (Fig. 1B). A more detailed time course analysis revealed that RANKL treatment of BMM increased the TBK1 protein level very rapidly in MVNP-containing BMM, with a twofold increase within 5 minutes and a fourfold increase at 60 minutes after RANKL addition, whereas in WT BMM the TBK1 protein level response to RANKL was slower and more modest (Fig. 1C). Of note, the serum starvation (2% FCS) and lack of M-CSF for 16 hours before addition of RANKL in this experimental protocol resulted in a smaller MVNP effect on TBK1 protein levels at time 0 compared with that observed in Fig. 1B. Together, these data indicate that MVNP increases TBK1 protein levels and potentiates the capacity of RANKL to further increase TBK1 protein.
We found that MVNP did not affect the levels of Tbk1 mRNA in NIH3T3 cells (Supplemental Fig. S2), so we examined if MVNP affected TBK1 protein stability. Treatment of EV-NIH3T3 and MVNP-NIH3T3 cells with the protein synthesis inhibitor cyclohexamide (CHX) revealed that MVNP prolonged the half-life of TBK1 protein from 2.5 to 3.5 hours (Fig. 1D, left panel). We then examined the stability of TBK1 protein in BMM from WT and MVNP mice. BMM cultured in the presence of RANKL for 24 hours were treated with CHX for the indicated times. TBK1 protein half-life was about 4 hours in early WT osteoclasts and was significantly prolonged in MVNP-expressing cells (Fig. 1D, right panel).
To confirm that there was more activated TBK1 in MVNP-containing cells, we analyzed the levels of phosphorylated-Ser172-TBK1 (P-TBK1) before and during TNF-α treatment of stably transduced MVNP-NIH3T3 and EV-NIH3T3 cells (Supplemental Fig. S3). Before TNF-α addition, both the phospho-TBK1/β-actin (Supplemental Fig. S3, left graph) and the total TBK1//β-actin (Supplemental Fig. S3, middle graph) levels were higher in cells expressing MVNP, indicating that the total amount of steady-state activated TBK1 was higher in the MVNP-containing cells. The phospho-TBK1/total TBK1 ratio (Supplemental Fig. S3, right graph) was increased rapidly by TNF-α in a time-dependent manner in both EV- and MVNP-NIH3T3 cells. Although the phospho-TBK1/total TBK1 ratio was similar in both cell types, total TBK1 and phospho-TBK1 were much higher in MVNP-NIH3T3 cells. These results indicate that the total amount of activated TBK1 was greater in the MVNP-NIH3T3 cells than in EV-NIH3T3 cells.
TBK1 overexpression stimulated IL-6 promoter activity and increased the endogenous expression of IL-6, p65 NF-κB, ATF7, and TAF12
We and others have shown that MVNP can increase IL-6 expression when introduced into cells.[23, 33] We recently reported that MVNP modulation of IL6 mRNA levels is via altered transcription rather than mRNA stabilization. Therefore, we determined if IL6 promoter regulation by MVNP could be mediated by TBK1 activity. We co-transfected expression constructs for TBK1, IKKϵ, and MVNP (or empty vector) with an IL6 promoter-luciferase reporter into HEK293 and NIH3T3 cells and assessed the relative activity of the transfected IL6 promoter. We found that the co-transfected IL6 promoter was activated by overexpressed TBK1, IKKϵ, or MVNP in both HEK293 and NIH3T3 cells (Fig. 2A). We then examined by qPCR analysis if transient transfection of TBK1, IKKϵ, and MVNP expression constructs stimulated the endogenous IL6 gene in NIH3T3 and RAW264.7 cells. We observed that, similar to the effects of MVNP expression, TBK1 and IKKϵ overexpression activated the endogenous IL6 gene in both cell types (Fig. 2B).
We previously reported that part of the pagetic phenotype induced by MVNP includes increases in the protein levels of the transcription factors TAF12, ATF7, and p65 NF-κB as well as increased phosphorylation of Ser536-p65 NF-κB.[1, 23, 26, 34] Comparison of stably transfected EV- and MVNP-RAW264.7 cells demonstrated that MVNP increased all three transcription factors and the amount of phospho-Ser536-p65 NF-κB (Fig. 2C, top panel). Consistent with the effects of transiently and stably expressed MVNP, both transient TBK1 and IKKϵ overexpression also increased phospho-Ser536-p65 NF-κB and total p65 NF-κB protein expression in RAW264.7 cells. However, only TBK1 overexpression mimicked the effect of MVNP in elevating ATF7 and TAF12 (Fig. 2C, bottom panel). We then compared the effects of transient expression of MVNP and TBK1 in HEK293 cells on p65 NF-κB, TAF12, and ATF7 proteins. Similar to its effects in osteoclast precursors, MVNP increased all three of these transcription factors as well as phospho-Ser536-p65 NF-κB (Fig. 2D). Unlike in RAW264.7 cells, TBK1 overexpression by itself in HEK293 cells dose-dependently increased p65 NF-κB (total and phospho-) and ATF7 but did not affect TAF12 protein levels (Fig. 2D). In summary, TBK1 overexpression increased IL6 reporter expression, endogenous IL6 mRNA levels, and phospho-Ser536-p65 NF-κB, total p65 NF-κB, ATF7, and TAF12 protein levels. These results indicate that TBK1 is a likely candidate for mediating many of the downstream effects of MVNP in the cell.
Overexpression of TBK1 in WT mouse BMM is not sufficient by itself to induce pagetic osteoclasts, but synergizes with MVNP
To discern if TBK1 overexpression in BMM is sufficient to induce a pagetic osteoclast phenotype, we infected BMM from both WT and TG-MVNP mice with a lentivirus expressing a hTBK1-GFP fusion protein. The GFP tag allowed us to visualize the virus infection efficiency and hTBK1 expression. After viral transduction, expression of hTBK1-GFP was detectable by fluorescence microscopy, and hTBK1-GFP mRNA expression was detectable by RT-PCR (Fig. 3A). However, we could not detect the hTBK1-GFP protein by Western blot of lysates from the BMM with either anti-TBK1 or anti-GFP, although Western blotting with both of these antibodies detected the hTBK1-GFP protein in lysates from HEK293 and MC4 cells infected with this lentivirus (Supplemental Fig. S4A). Cotransfection of the lentiviral plasmid encoding the hTBK1-GFP protein activated an IL6-promoter reporter in HEK293 cells, indicating that the hTBK1-GFP protein was functional (Supplemental Fig. S4B). The above results suggest that the transduced BMM are expressing low levels of the exogenous hTBK1-GFP protein and/or that there is more proteolytic degradation of the protein during isolation from BMM resulting in lysate levels that are below the detection limits of Western blotting. TRAP staining after 4 days of RANKL treatment revealed that this low level of exogenous TBK1 protein was not sufficient by itself to increase the number of TRAP+ multinuclear osteoclasts formed. However, it was able to synergize with MVNP to significantly increase osteoclast number (∼threefold) compared with MVNP alone (Fig. 3B). Similar results were obtained for the production of mouse IL6 mRNA (Fig. 3C). Importantly, the transduction of TBK1 in WT cells increased the mRNA levels of Tnfrsf11a (RANK) by threefold and cathepsin K (Ctsk) by twofold (Fig. 3D). The transduced TBK1 synergized with MVNP to increase all three mRNAs. Interestingly, IL6 and Ctsk mRNA were increased ∼threefold over MVNP alone (similar to osteoclast number), whereas RANK mRNA was increased 21-fold. These results indicate that increasing TBK1 by itself did not recapitulate the effects of MVNP, and the synergy with MVNP suggests that MVNP activation of TBK1 is important.
Knockdown of TBK1 with a shRNA lentivirus impaired MVNP's capacity to increase IL-6 secretion, as well as TAF12 and ATF7 protein expression
To test whether TBK1 is necessary for MVNP's modulation of IL-6 expression and other aspects of the pagetic osteoclast phenotype, we first tested the effects of a pharmacological catalytic activity inhibitor of TBK1/IKKϵ, BX795, on IL6 mRNA expression by EV- and MVNP-NIH3T3 cells. BX795 dose-dependently inhibited the MVNP-induced increase in steady-state IL6 mRNA (Fig. 4A). We also investigated if decreasing TBK1 amount or activity in HEK293 cells blocked the effects of transfected MVNP by co-transfecting MVNP with a shTBK1 plasmid and a dominant-negative TBK1 (DN-TBK1) expression vector, which is a kinase-inactive TBK1(K38A) mutant. These results showed that MVNP increased phospho-p65 NF-κB, total p65 NF-κB, and ATF7 protein levels (Fig. 4B). Co-transfection with either DN-TBK1 or shTBK1 blocked these MVNP-induced changes (Fig. 4B). Together these results indicate that MVNP requires TBK1 signal transduction for at least some of the observed effects of MVNP expression.
Infection of MVNP-NIH3T3 cells with two different shTBK1 lentivirus clones decreased the levels of TBK1, ATF7, and TAF12 protein, although shTBK1 lentivirus #2 was more effective (Fig. 4C). Therefore, we utilized shTBK1 lentivirus #2 for all our additional studies. EV- and MVNP-NIH3T3 cells were transduced with both scrambled control (SCR) and shTBK1 lentiviruses. Puromycin was added at 6 days post-infection and thereafter. At 38 days post-infection, TBK1 protein expression levels were assessed by Western blotting, which revealed that the MVNP-increased TBK1 protein was significantly decreased by the shTBK1 (Fig. 4D, lower band in doublet). ELISA analysis of cell culture supernatants revealed that secreted IL-6 protein levels were equivalent in supernatants from SCR and shTBK1 lentivirus-transduced EV-NIH3T3 cells, indicating that TBK1 signaling does not regulate the basal production of IL-6 in these cells (Fig. 4E). As expected, there was much higher IL-6 in the supernatants from SCR lentivirus-transduced MVNP-NIH3T3 cells than in shTBK1 lentivirus-transduced EV-NIH3T3 cells. Most importantly, TBK1 downregulation by shTBK1 lentivirus decreased the ability of MVNP to increase IL-6 so dramatically that the MVNP-NIH3T3 cells produced only slightly more than the EV-NIH3T3 cells (Fig. 4E).
Knockdown of TBK1 in MVNP-expressing osteoclast progenitors impaired the increase in osteoclast formation induced by RANKL and TNF-α
To determine whether TBK1 is required for MVNP induction of a pagetic osteoclast phenotype, we first treated BMM from WT and TRAP-MVNP mice with the TBK1/IKKϵ inhibitor BX795 and analyzed the effect on IL6 gene expression. Consistent with our results in NIH3T3 cells (Fig. 4A), the MVNP-elevated IL6 mRNA expression was dose-dependently inhibited by BX795 (Fig. 5A). We then used the shTBK1 and SCR lentiviruses to infect, aided by magnetofection, the nonadherent BMM from WT and TG-MVNP mice. The shTBK1 lentivirus markedly reduced the TBK1 protein in both WT and TG-MVNP cells after 1 day RANKL exposure (Fig. 5B). TRAP staining of RANKL-treated BMM showed that MVNP still increased the osteoclast number obtained from BMM infected with the SCR lentivirus when compared with WT. Importantly, TBK1 knockdown blocked the increased osteoclast formation from TG-MVNP BMM (Fig. 5C and Supplemental Fig. S5A). The SCR lentivirus did not prevent MVNP from increasing the mRNA levels of IL6 (Fig. 5D) and the osteoclast marker genes RANK and Ctsk (Fig. 5E). The shTBK1 lentivirus in WT osteoclasts did not affect the mRNA levels of IL6 and the osteoclast marker gene RANK but was slightly inhibitory for expression of Ctsk mRNA. Importantly, the shTBK1 lentivirus strongly decreased the MVNP effects on expression of IL6, RANK, and Ctsk. To confirm these effects are not caused by a variation in transduction efficiency, we did qPCR to assay the mRNA levels of the puromycin resistance gene (Puro) expressed by the lentiviruses used and found similar levels in each of the groups, indicating that the transduction efficiencies in the four cell populations were similar (Supplemental Fig. S5B).
A similar experiment with TNF-α revealed that knockdown of TBK1 also impaired the MVNP-induced increased response of osteoclast progenitors to TNF-α, completely blocking the MVNP effects on osteoclast formation, as well as IL6, RANK, and Ctsk mRNA expression (Supplemental Fig. S6). These results demonstrate that there is an even tighter requirement for TBK1 function for MVNP to induce pagetic osteoclasts in response to TNF-α treatment than observed with RANKL. Together, these data demonstrate that TBK1 mediates the MVNP-induced increased response of osteoclast progenitors to RANKL and TNF-α.
Osteoclasts expressing MVNP display a pagetic phenotype.[1, 10, 11] We found that MVNP activates NF-κB, increases IL-6 production, and induces pagetic osteoclast formation through an alternative pathway that involves activation of TBK1. The presence of MVNP increased TBK1 in the four cell types examined (RAW264.7, NIH3T3, HEK293, and primary BMM) and TBK1 activation as reflected in the increased amount of phosphorylated-Ser172-TBK1. Overexpression of TBK1 in the cell lines increased expression of both co-transfected IL6 promoter luciferase reporters and the endogenous IL6 gene, indicating that the large increase in TBK1 promoted auto-activation of TBK1 that resulted in activation of the IL6 gene. Increased expression of IL-6 is not only a marker of a pagetic osteoclast but also is required for MVNP-expressing mice to form pagetic osteoclasts and bone lesions and is not induced by the p62P392L mutant.[1, 2, 10, 11] Inhibition of TBK1 by BX795 or knockdown of TBK1 by shTBK1 lentivirus blocked the capacity of MVNP to upregulate IL6 gene expression in both NIH3T3 cells and primary BMM, as well as blocked the MVNP-induced increase in osteoclast numbers after RANKL or TNF-α stimulation. Interestingly, the presence of IKKϵ in the TBK1 knockdown cells could not compensate for the loss of TBK1, suggesting that TBK1 and IKKϵ are not functionally redundant in their capacity to transduce the MVNP signal to regulate IL6 expression and osteoclast formation. However, these data do not indicate that IKKϵ has no role in osteoclasts. RANKL rapidly upregulated both TBK1 and IKKϵ protein levels in BMM. Interestingly, we did not observe an effect of TBK1 knockdown on the osteoclast differentiation of BMM from WT mice. This may indicate that IKKϵ can compensate for loss of TBK1 during normal osteoclastogenesis. Together these data demonstrate that MVNP activation of TBK1 is required for upregulation of IL6 expression and formation of pagetic osteoclasts (Fig. 6).
TBK1/IKKϵ have been reported to activate both IRF3/IRF712 and NF-κB[17, 19, 21] and possibly both C/EBPβ and C/EBPδ. We focused on NF-κB in this study because we have shown that MVNP increases both total p65 NF-κB and phospho-Ser536-p65 NF-κB, and NF-κB is a crucial regulator of IL6 gene expression. We confirmed that TBK1 upregulates p65 NF-κB in RAW264.7 cells and in HEK293 cells. More significantly, use of a dominant-negative TBK1 or TBK1 knockdown in HEK293 cells blocked the MVNP-induced increase in both total p65 NF-κB and phospho-Ser536-p65 NF-κB, suggesting that MVNP requires TBK1 to enhance NF-κB activity and thereby increase IL6 transcription.
We have recently published that MVNP can induce phosphorylation of the transcription factor FoxO3, leading to its proteolysis and to a decrease in the expression of its target Sirtuin 1 (Sirt1), an NF-κB deacetylase, thereby increasing NF-κB activity and IL6 expression.[10, 23] Among the kinases reported to phosphorylate FoxO3 are AKT and IKKϵ.[37-39] Further, TBK1 and IKKϵ have been reported to activate AKT.[40, 41] Our preliminary data (not shown) revealed that overexpression of TBK1 did not decrease Sirt1 and loss of TBK1 activity did not rescue Sirt1 expression from MVNP repression. These data indicate that MVNP does not act via TBK1 to regulate Sirt1 expression. Therefore, both decreased Sirt1 activity[10, 23] and increased TBK1 activity are required for MVNP to increase IL6 gene expression (Fig. 6).
TAF12, a VDR co-activator that plays an important role in the increased osteoclast formation in Paget's disease by increasing the sensitivity of osteoclast precursors to 1,25-(OH)2D3, is increased by the presence of MVNP in the cell.[26, 42, 43] We recently reported that MVNP increases ATF7, which interacts with TAF12, and that ATF7 increases TAF12 expression and contributes to the hypersensitivity of osteoclast precursors to 1,25-(OH)2D3 in Paget's disease. Therefore, we assessed whether TBK1 mediates MVNP regulation of TAF12 and/or ATF7. In both RAW264.7 and HEK293 cells, TBK1 overexpression increased ATF7, but only in RAW264.7 cells did we observe increased TAF12. In the HEK293 cells, a dominant-negative TBK1 or TBK1 knockdown repressed the MVNP-induced increase of ATF7. TBK1 knockdown decreased both ATF7 and TAF12 in MVNP-NIH3T3 cells. These data indicate that MVNP increases ATF7 via TBK1 activity in all three cell types. However, MVNP acts through TBK1 to elevate TAF12 in both RAW264.7 and NIH3T3 cells but not in HEK293 cells, perhaps reflecting cell-type differences. Because little is known about how TAF12 and ATF7 expression is regulated, we do not know if TBK1 activation of NF-κB or another transcription factor is involved in elevating ATF7 and TAF12 expression.
Protein stability assays showed that MVNP slowed TBK1 degradation. TBK1 stability was reported to be regulated by the presence of K63-linked versus K48-linked polyubiquitination, which is dynamically altered by a number of E3 ligases, such as K63 E3 ligases MIB1 and 2, K48 E3 ligase TRIP, and de-ubiquinylation enzyme A20 (with the help of TAX1BP1).[46, 47] Which E3 ligases or DUBs are differentially recruited in the presence of MVNP remains to be elucidated. RANKL more rapidly upregulated TBK1 protein in the presence of MVNP, with a ∼twofold increase within 5 minutes, followed by a continual increase through 60 minutes. The rapidity of the change suggests that RANKL potentiates the MVNP stabilization of TBK1 or increases translation.
Infection of BMM from WT and TRAP-MVNP mice with a lentivirus expressing a hTBK1-GFP fusion protein resulted in potentiation of the MVNP effects on osteoclast formation and IL-6 expression but did not alter these in WT cells, suggesting that the low level of ectopic hTBK1-GFP protein in these cells was not able to trans-autophosphorylate and auto-activate and required MVNP-induced activation. However, the ectopic hTBK1-GFP levels were capable of increasing RANK and Ctsk mRNA above EV in WT cells after 4 days of RANKL induction. Together these results indicate TBK1 overexpression by itself is not sufficient to induce aberrant osteoclast formation but requires either activation by MVNP or a partner that is altered by MVNP.
Our data indicate that MVNP-induced activation of TBK1 and the consequent increased IL-6 production via enhanced NF-κB are important for the generation of pagetic osteoclasts. However, activated TBK1 and IKKϵ have several other functions that may also contribute to the generation of pagetic osteoclasts. TBK1 has been reported to be involved in regulating autophagic maturation and to phosphorylate p62 at Ser403 in the ubiquitin-binding domain, thereby enhancing binding and clearance. TBK1 also is critical for K-Ras-dependent non-small lung cancer cells because it drives basal autophagy via the cargo receptors NDP52 and TAX1BP1, which promotes noncanonical NF-κB signaling. This may be another pathway by which MVNP regulation of TBK1 enhances NF-κB activation of the IL6 gene. Both TBK1 and IKKϵ can activate AKT via a PI3K-indendent pathway and a PI3K-dependent pathway. The PI3K/AKT signaling pathway plays a critical role in activating osteoclast differentiation and bone resorption in normal and diseased states. Increased osteoclast survival may contribute to the pagetic osteoclast phenotype. Of great interest is that TBK1 binds optineurin (OPTN), a NEMO-like IKK family member, that both regulates TBK1 activity and is phosphorylated by TBK1 on Ser177, which enhances LC3-binding affinity and promotes autophagy.[53, 54] Genome-wide association studies have identified variants in the OPTN gene as a genetic risk factor for Paget's disease, although the variations are not in the coding region. It remains to be elucidated if MVNP alters the interaction of TBK1 and OPTN.
Our data from both cell lines and mouse BMM demonstrated that TBK1 plays a critical role in mediating the effects of MVNP on the expression of p65 NF-κB, TAF12, ATF7, and IL-6, key contributors to the pagetic osteoclast phenotype. It has been shown that TBK1 and IKKϵ are potential therapeutic targets in rheumatoid arthritis.[56, 57] Therapeutics that inhibit TBK1 activity, like the inhibitor BX795, and other more specific inhibitors that are in the pipeline[58, 59] may prevent the development of Paget's disease of bone.
GDR is a consultant to Amgen and develops CME materials for Clinical Care Options. All other authors state that they have no conflicts of interest.
The authors gratefully thank Dr Tom Maniatis for TBK1 and IKKϵ plasmids; Dr Jian Zhang for the IL6 promoter reporters; Dr Guoshuang Xu for FUGW, RSV-REV, and PRRE; the UPCI Lentivirus Facility for the GFP, SCR, and shTBK1 lentiviruses; and the Veterans Administration Pittsburgh Healthcare System, Research and Development for use of the facilities. We thank other members of our laboratories for helpful discussion.
Support for the UPCI Lentiviral Facility was provided by the Cancer Center Support Grant from the National Institutes of Health (P30 CA047904). This work was supported by the National Institutes of Health (NIAMS grants AR057310 to DLG and AR057308 to GDR) and Research Funds from the Veterans Administration (to GDR). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIAMS, NIH, the Department of Veterans Affairs, or the United States Government.
Authors' roles: Study design: QS, GDR, and DLG. Study conduct: QS, BS, FW, NK, JJW, and DLG. Data collection: QS and BS. Developed and provided the transgenic mice: JJW. Created the EV and MVNP-RAW264.7 cell lines: NK. Data analysis: QS and DLG. Data interpretation: QS, GDR, and DLG. Drafting manuscript: QS and DLG. Revising manuscript content: QS, FW, JJW, GDR, and DLG. Approving final version of manuscript: all authors. DLG takes responsibility for the integrity of the data analysis.