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Additional Supporting Information may be found in the online version of this article.

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Fig. S1 Analysis of the effects (mean ± SEM) Scl-Ab at 30, 50 and 100 mg/Kg/Wk IV on spine trabecular bone volume (BV/TV) as measured by μCT scanning in WT mice. n = 10 mice per group. *** = p < 0.001 compared to controls.

Fig. S2 Flow cytometric analysis of splenocytes harvested at sacrifice from WT mice, TCRβ−/− mice, and TCRβ−/− mice subjected to adoptive transfer of WT T or Wnt10b−/− T cells 3 weeks before sacrifice. The adoptive transfer of T cells was carried out by injecting in the tail vein 5 × 106 T cells purified by negative immunomagnetic selection.

Fig. S3 Analysis of the effects (mean ± SEM) of iPTH, Scl-Ab and combined treatment on spine trabecular thickness (Tb.Th) as measured by μCT scanning in WT mice (A), TCRβ−/− mice (B), TCRβ−/− mice previously subjected to adoptive transfer of WT T cells (C) or Wnt10b−/− T cells (D) and global Wnt10b−/− mice (E). (n = 10 mice per group). * = p < 0.05, ** = p < 0.01 and *** = p < 0.001 compared to the indicated group.

Fig. S4 Analysis of the effects (mean ± SEM) of iPTH, Scl-Ab and combined treatment on spine trabecular number (Tb.N) as measured by μCT scanning in WT mice (A), TCRβ−/− mice (B), TCRβ−/− mice previously subjected to adoptive transfer of WT T cells (C) or Wnt10b−/− T cells (D) and global Wnt10b−/− mice (E). (n = 10 mice per group). * = p < 0.05, ** = p < 0.01 and *** = p < 0.001 compared to the indicated group.

Fig. S5 Analysis of the effects (mean ± SEM) of iPTH, Scl-Ab and combined treatment on spine connectivity density (Conn.D) as measured by μCT scanning in WT mice (A), TCRβ−/− mice (B), TCRβ−/− mice previously subjected to adoptive transfer of WT T cells (C) or Wnt10b−/− T cells (D) and global Wnt10b−/− mice (E). (n = 10 mice per group). * = p < 0.05, ** = p < 0.01 and *** = p < 0.001 compared to the indicated group.

Fig. S6 Analysis of the effects (mean ± SEM) of iPTH, Scl-Ab and combined treatment on femoral cortical thickness (Co.Th) as measured by μCT scanning in WT mice (A), TCRβ−/− mice (B), TCRβ−/− mice previously subjected to adoptive transfer of WT T cells (C) or Wnt10b−/− T cells (D) and global Wnt10b−/− mice (E). (n = 10 mice per group). * = p < 0.05, ** = p < 0.01 and *** = p < 0.001 compared to the indicated group.

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