Osterix and NO66 Histone Demethylase Control the Chromatin of Osterix Target Genes During Osteoblast Differentiation
Version of Record online: 19 MAR 2014
© 2014 American Society for Bone and Mineral Research
Journal of Bone and Mineral Research
Volume 29, Issue 4, pages 855–865, April 2014
How to Cite
Sinha, K. M., Yasuda, H., Zhou, X. and deCrombrugghe, B. (2014), Osterix and NO66 Histone Demethylase Control the Chromatin of Osterix Target Genes During Osteoblast Differentiation. J Bone Miner Res, 29: 855–865. doi: 10.1002/jbmr.2103
- Issue online: 19 MAR 2014
- Version of Record online: 19 MAR 2014
- Accepted manuscript online: 23 SEP 2013 07:33AM EST
- Manuscript Accepted: 22 AUG 2013
- Manuscript Revised: 7 AUG 2013
- Manuscript Received: 20 AUG 2012
- NIH NIAMS. Grant Numbers: AR49072, AR061590, CA16672
Additional Supporting Information may be found in the online version of this article.
Suppl. 1. Levels of endogenous Bsp by immunostaining. Osx-null calvarial cells transfected with Flag-HA-Osx for 24h were fixed with 4% formaldehyde for 20min at room temperature, permeabilized with 0.1% triton X-100 for 10min, blocked with 3%BSA and then incubated with primary antibodies anti-HA (A) and anti-Bsp (B) for 1 hour in humidified chamber followed by detection with alexa-fluor conjugated secondary antibodies. Cells were visualized under microscope with 40X objective. Panel C indicates DAPI staining to visualize nuclei, and Panel D indicates a merged of three colors.
Suppl. 2 and 3. DNA methylation in the Bsp promoter persists in Osx-null calvaria. Sequencing chromatogram of PCR products of region II and III amplified from bisulfite-treated DNA isolated from ES cells, Osx-wt and Osx-null calvaria of embryos E18.5. Arrow indicates methylated cytosines (8-14) undergo DNA demethylation in wild type when compared with that in ES cells and Osx-null calvaria.
Primers sequence used in this study
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