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jbmr2108-sm-0001-SupplementalFigure-S1.tif54KFigure S1. Total RNA was extracted from femora of 2 RelB-/- and 2 WT littermate (1 and 2, representing each mouse) 9-week-old mice using Trizol reagents. mRNA expression levels of ALP, osteocalcin (OCal), Runx2, RANKL, OPG were tested by real-time PCR and the RANKL/OPG expression ratio was calculated.

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