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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
jbmr2159-sm-0001-sm-SuppFigure-S1.tif181KSupporting Figure 1. Vertebral trabecular bone is reduced in Osx1Cre.gp130f/f male mice. Vertebrae from 12 week-old male Osx1Cre.gp130 mice were scanned by microCT for trabecular bone volume/tissue volume (BV/TV), trabecular number (TbN), trabecular thickness (TbTh), and trabecular spacing (TbSp) (n = 8 w/w, n = 8 w/f, n = 7 f/f). Data is mean+SEM and **p < 0.01, ***p < 0.001.
jbmr2159-sm-0002-sm-SuppFigure-S2.tif2320KSupporting Figure 2. Trabecular bone is reduced in male mice with gp130 deletion throughout the osteoblast lineage. Proximal tibial metaphyseal trabecular bone structure assessed by histomorphometry in 6, 12, and 26 week-old male Osx1Cre.gp130 mice for (A) % bone volume/tissue volume (BV/TV), (B) trabecular thickness (TbTh), (C) trabecular number (TbN), and (D) trabecular spacing (TbSp). Von Kossa images (E) represent the average values for 12 week-old trabecular bone structure in (A-D) (6 week-old mice: n = 8 w/w, n = 6 f/f ; 12 week-old mice: n = 15 w/w, n = 13 f/f; 26 week-old mice: n = 4 w/w, n = 6 f/f). Data is mean+SEM and *p < 0.05, ***p < 0.001, ****p < 0.0001.
jbmr2159-sm-0003-sm-SuppFigure-S3.tif171KSupporting Figure 3. Vertebral trabecular bone is reduced with osteocyte gp130 deletion. Vertebral trabecular bone volume/tissue volume (BV/TV), trabecular number (TbN), trabecular thickness (TbTh), and trabecular spacing (TbSp) in 12 week-old male DMP1Cre.gp130 mice by microCT (n = 8 w/w, n = 9 f/f). Data is mean+SEM and ***p < 0.001, ****p < 0.0001.
jbmr2159-sm-0004-sm-SuppFigure-S4.tif238KSupporting Figure 4. gp130 deletion in osteocytes reduces trabecular bone mass. Trabecular bone structure of male 12 and 26 week-old DMP1Cre.gp130 mice assessed by histomorphometry. (A) % bone volume/tissue volume (BV/TV), (B) trabecular thickness (TbTh), (C) trabecular number (TbN), and (D) trabecular spacing (TbSp) in the metaphysis of the proximal tibia. Von Kossa images (E) represent the average value for 12 week-old trabecular bone structure in (A-D) (12 week-old mice: n = 9 w/w, n = 11 f/f; 26 week-old mice: n = 8 w/w and n = 8 f/f). Data is mean+SEM and **p < 0.01, ***p < 0.001.
jbmr2159-sm-0005-sm-SuppFigure-S5.tif160KSupporting Figure 5. Femoral lengths at 12 and 26 weeks are not altered with gp130 deletion in the Osx1Cre or DMP1Cre strain. 12 week-old mice: Osx1Cre (n = 8 w/w, n = 7 f/f), DMP1Cre (n = 8 w/w, n = 12 f/f); 26 week-old mice: Osx1Cre (n = 8 w/w, n = 8 f/f), DMP1Cre (n = 8 w/w, n = 8 f/f). Data is mean+SEM.
jbmr2159-sm-0006-sm-SuppFigure-S6.tif127KSupporting Figure 6. Mineral density and microstructure properties and are not altered with gp130 deletion. (A) MicroCT analysis of tissue mineral density (TMD) in 26 week-old DMP1Cre.gp130 mice (n = 9 w/w, n = 8 f/f). (B) Femora from 12 week-old male (n = 5 w/w, n = 9 f/f) and female (n = 5 w/w, n = 5 f/f) DMP1Cre.gp130 mice assessed by Reference Point Indentation (10 cycles, max force = 2N). Shown are total indentation distance (TID) of the probe, average loading slope, average unloading slope, and indentation distance increase (IDI). Data is mean+SEM.

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