Altered Bone Material Properties in HLA-B27 Rats Include Reduced Mineral to Matrix Ratio and Altered Collagen Cross-Links

Authors

  • Sonja Gamsjaeger,

    1. Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Vienna, Austria
    Search for more papers by this author
  • Apurva K Srivastava,

    1. Laboratory of Human Toxicology and Pharmacology, Applied/Developmental Research Directorate, Frederick National Laboratory for Cancer Research, operated by Leidos Biomed for the National Cancer Institute, Frederick, MD, USA
    Search for more papers by this author
  • Jon E Wergedal,

    1. Musculoskeletal Disease Center, Jerry L Pettis VA Medical Center, Loma Linda, CA, USA
    Search for more papers by this author
  • Jochen Zwerina,

    1. Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Vienna, Austria
    Search for more papers by this author
  • Klaus Klaushofer,

    1. Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Vienna, Austria
    Search for more papers by this author
  • Eleftherios P Paschalis,

    Corresponding author
    1. Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Vienna, Austria
    • Address correspondence to: Eleftherios P Paschalis, PhD, Ludwig Boltzmann Institute of Osteology, Hanusch Krankenhaus, Heinrich Collin Str. 30, A-1140 Vienna, Austria. E-mail: eleftherios.paschalis@osteologie.at

    Search for more papers by this author
  • Dimitris N Tatakis

    1. Division of Periodontology, College of Dentistry, The Ohio State University, Columbus, OH, USA
    Search for more papers by this author

ABSTRACT

Spondyloarthropathy and inflammatory bowel disease (IBD), which includes ulcerative colitis and Crohn's disease, are often associated with severe osteopenia/osteoporosis in both children and adults. HLA-B27 transgenic rats present a phenotype that includes severe colitis and severely accelerated alveolar bone loss. The purpose of this study was to evaluate long bone density status, systemic bone metabolic markers, and intrinsic bone material properties in HLA-B27 transgenic (TG) rats, and compare them with those of age- and sex-matched wild-type (WT) animals. The results indicate that in the HLA-B27 rat, an animal susceptible to both alveolar bone loss (ABL) and long bone osteopenia, there is a statistically significant negative correlation between ABL and long bone bone mineral density (BMD), as well as mineral/matrix ratio at active bone-forming trabecular surfaces. The TG animals had a lower mineral/matrix ratio and higher relative proteoglycan and advanced glycation end product (ϵ-N-Carboxymethyl-L-lysine) content and pyridinoline/divalent collagen cross-link ratio compared with WT. These results may provide better understanding of the interrelationship between osteoporosis and oral bone loss, the underlying causes of the inferior bone strength in the HLA-B27 transgenic animals, and could prove to be a useful model in the elucidation of the pathophysiology of spondyloarthropathy and IBD-associated osteopenia/osteoporosis and in the evaluation of pharmacological intervention(s) against such conditions. © 2014 American Society for Bone and Mineral Research.

Ancillary