Research Article

Cloning, sequencing, and characterization of CYP1A1 cDNA from leaping mullet (Liza Saliens) liver and implications for the potential functions of its conserved amino acids

  1. Alaattin Sen1,*,
  2. Chin-Hwa Hu1,†,
  3. Ena Urbach2,
  4. Jun-Lan Wang-Buhler1,
  5. Yea-Huey Yang1,
  6. Emel Arinc3,
  7. Donald R. Buhler1,4,5

Article first published online: 18 OCT 2001

DOI: 10.1002/jbt.10005

Issue

Journal of Biochemical and Molecular Toxicology

Journal of Biochemical and Molecular Toxicology

Volume 15, Issue 5, pages 243–255, 2001

Additional Information

How to Cite

Sen, A., Hu, C.-H., Urbach, E., Wang-Buhler, J.-L., Yang, Y.-H., Arinc, E. and Buhler, D. R. (2001), Cloning, sequencing, and characterization of CYP1A1 cDNA from leaping mullet (Liza Saliens) liver and implications for the potential functions of its conserved amino acids. Journal of Biochemical and Molecular Toxicology, 15: 243–255. doi: 10.1002/jbt.10005

Author Information

  1. 1

    Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis Oregon, USA

  2. 2

    Department of Microbiology, Oregon State University, Corvallis Oregon, USA

  3. 3

    Department of Biological Sciences, Middle East Technical University, 06531 Ankara, Turkey

  4. 4

    Environmental Health Sciences Center, Oregon State University, Corvallis Oregon, USA

  5. 5

    Marine/Fresh Water Biomedical Center, Oregon State University, Corvallis Oregon, USA

  1. †

    On leave from Institute of Marine Biotechnology, Taiwan National Ocean University, Keelung, Taiwan, Republic of China

*Department of Biology, Pamukkale University, Kinikli-Denizli, Turkey

Publication History

  1. Issue published online: 18 OCT 2001
  2. Article first published online: 18 OCT 2001
  3. Manuscript Accepted: 18 JUN 2001
  4. Manuscript Revised: 7 JUN 2001
  5. Manuscript Received: 2 APR 2001

Funded by

  • National Institutes of Health, USA. Grant Number: ES000210, ES03850, and ES04766

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Keywords:

  • CYP1A1;
  • Leaping Mullet;
  • Liza Saliens;
  • Phylogenetic Analysis;
  • Cloning

Abstract

A 2,037 bp CYP1A1 cDNA (GenBank AF072899) was cloned through screening of a λZipLox cDNA library constructed from the liver of a leaping mullet (Liza saliens) fish captured from Izmir Bay on the Aegean coast of Turkey using rainbow trout CYP1A1 cDNA as a probe. This clone has a 130 bp 5'-flanking region, a 1,563 bp open reading frame (ORF) encoding a 521-amino acid protein (58,972 Da), and a 344 bp 3'-untranslated region without a poly (A) tail. Alignment of the deduced amino acids of CYP1A1 cDNAs showed 58% and 69–96% identities with human and 12 other fish species, respectively. Southern blot analysis suggested that this CYP1A1 cDNA was from a single-copy gene. Based on the comparison with CYP1A1 genes reported for fish and mammals, the leaping mullet CYP1A1 gene is probably split into 7 exons. The intron insertion sites were predicted. Alignment of the CYP1A1 cDNA encoded amino acids from 13 fish and 7 mammalian species disclosed differences in highly conserved amino acids between aquatic and land vertebrates. The possible associated secondary structure; conserved motifs and substrate-binding sites were discussed. The phylogenetic relationships of CYP1A1s among 13 fish species were analyzed by a distance method. © 2001 John Wiley & Sons, Inc. J Biochem Mol Toxicol 15:243–255, 2001

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