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Biochemical Mini-Review
The roles of cytochrome b5 in cytochrome P450 reactions
Article first published online: 12 DEC 2002
DOI: 10.1002/jbt.10052
Copyright © 2002 Wiley Periodicals, Inc.
Issue
1099-0461/asset/cover.gif?v=1&s=464a877af403ab8bb706d1be0c08d11ee0c6d3f2 "Journal of Biochemical and Molecular Toxicology")
Journal of Biochemical and Molecular Toxicology
Volume 16, Issue 6, pages 311–316, December 2002
Additional Information
How to Cite
Porter, T. D. (2002), The roles of cytochrome b5 in cytochrome P450 reactions. Journal of Biochemical and Molecular Toxicology, 16: 311–316. doi: 10.1002/jbt.10052
Publication History
- Issue published online: 12 DEC 2002
- Article first published online: 12 DEC 2002
- Manuscript Revised: 26 AUG 2002
- Manuscript Accepted: 26 AUG 2002
- Manuscript Received: 12 AUG 2002
- Abstract
- References
- Cited By
Keywords:
- Cytochrome b5;
- Cytochrome P450;
- Cytochrome b5 Reductase;
- Cytochrome P450 Reductase;
- Electron Transfer
Abstract
Cytochrome b5, a 17-kDa hemeprotein associated primarily with the endoplasmic reticulum of eukaryotic cells, has long been known to augment some cytochrome P450 monooxygenase reactions, but the mechanism of stimulation has remained controversial. Studies in recent years have clarified this issue by delineating three pathways by which cytochrome b5 augments P450 reactions: direct electron transfer of both required electrons from NADH–cytochrome b5 reductase to P450, in a pathway separate and independent of NADPH–cytochrome P450 reductase; transfer of the second electron to oxyferrous P450 from either cytochrome b5 reductase or cytochrome P450 reductase; and allosteric stimulation of P450 without electron transfer. Evidence now indicates that each of these pathways is likely to operate in vivo. © 2002 Wiley Periodicals, Inc. J Biochem Mol Toxicol 16:311–316, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.10052