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Al2O3 Nanoparticles Induce Mitochondria-Mediated Cell Death and Upregulate the Expression of Signaling Genes in Human Mesenchymal Stem Cells

Authors

  • Ali A. Alshatwi,

    Corresponding author
    • Nanobiotechnology and Molecular Biology Research Lab, Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia
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  • Periasamy Vaiyapuri Subbarayan,

    1. Nanobiotechnology and Molecular Biology Research Lab, Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia
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  • E. Ramesh,

    1. Nanobiotechnology and Molecular Biology Research Lab, Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia
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  • Amal A. Al-Hazzani,

    1. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
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  • Mohammed A. Alsaif,

    1. College of Applied Medical Sciences, King Saud University, Riyadh 11451, Saudi Arabia
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  • Abdulrahman A. Alwarthan

    1. Departments of Chemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
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Correspondence to: Ali A. Alshatwi.

ABSTRACT

An increase in the broad usage of Al2O3 nanoparticles (ANPs) in the food and agricultural sectors may produce rare hazards for human health. The objective of this study was to assess the acute toxicity of ANPs in human mesenchymal stem cells (hMSCs) in vitro. Cell viability, cellular uptake, morphology, and gene expression using quantitative real-time polymerase chain reaction (qRT-PCR) were analyzed. The results indicate that ANPs have a significant and dose-dependent effect on cytotoxicity. Control cells showed a characteristic, homogeneous nuclear staining pattern, whereas ANP-exposed cells showed abnormal nuclear morphological changes such as condensation or fragmentation. An early characteristic of apoptosis was observed in ANP-treated cells. Further confirmation of cell death in hMSCs was observed through increased expression of chosen signaling genes and also decreased expression of Bcl-2 during mitochondria-mediated cell death. Although they provide great advantages in food and agricultural products, the chronic and acute toxicity of ANPs still needs to be assessed carefully. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:469-476, 2012; View this article online at wileyonlinelibrary.com. DOI 10:1002/jbt.21448

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