This article was originally published on 21November 2012. The list of corresponding authorswas incomplete due to a publisher error. This was corrected on 6 February 2013.
Effects of siRNA Targeting c-Myc and VEGF on Human Colorectal Cancer Volo Cells
Article first published online: 21 NOV 2012
© 2012 Wiley Periodicals, Inc.
Journal of Biochemical and Molecular Toxicology
Volume 26, Issue 12, pages 499–505, December 2012
How to Cite
Tai, J., Wang, G., Liu, T., Wang, L., Lin, C. and Li, F. (2012), Effects of siRNA Targeting c-Myc and VEGF on Human Colorectal Cancer Volo Cells. J. Biochem. Mol. Toxicol., 26: 499–505. doi: 10.1002/jbt.21455
- Issue published online: 20 DEC 2012
- Article first published online: 21 NOV 2012
- Manuscript Accepted: 7 OCT 2012
- Manuscript Revised: 29 SEP 2012
- Manuscript Received: 2 AUG 2012
- Colorectal Cancer;
c-Myc and vascular endothelial growth factor (VEGF) genes are frequently deregulated and overexpressed in this malignancy, and strategies designed to inhibit c-Myc and VEGF expression in cancer cells may have considerable therapeutic value. In the present study, we design and use short interfering RNA (siRNA) to inhibit c-Myc and VEGF expression in colorectal cancer Volo cells and validate their effects on cell proliferation, cell cycle, apoptosis, and cell metastasis. Upon transient transfection with plasmid-encoding siRNA, it was found that expression of c-Myc and VEGF was significantly downregulated in siRNA-transfected cells and the downregulation of c-Myc and VEGF inhibited cell growth and induced apoptosis and metastasis of Volo cells. c-Myc and VEGF downregulation also increased cell population in the G0–G1 phase. In conclusion, the specific siRNA efficiently silenced the expression of c-Myc and VEGF, further suppressed the cell proliferation, triggered cell apoptosis, and inhibited cell invasiveness of colorectal cancer Volo cells. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:499-505, 2012;Viewthis article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21455