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Effects of siRNA Targeting c-Myc and VEGF on Human Colorectal Cancer Volo Cells

Authors


  • This article was originally published on 21November 2012. The list of corresponding authorswas incomplete due to a publisher error. This was corrected on 6 February 2013.

Correspondence to: L. Wang, F. Li, or C. Lin.

ABSTRACT

c-Myc and vascular endothelial growth factor (VEGF) genes are frequently deregulated and overexpressed in this malignancy, and strategies designed to inhibit c-Myc and VEGF expression in cancer cells may have considerable therapeutic value. In the present study, we design and use short interfering RNA (siRNA) to inhibit c-Myc and VEGF expression in colorectal cancer Volo cells and validate their effects on cell proliferation, cell cycle, apoptosis, and cell metastasis. Upon transient transfection with plasmid-encoding siRNA, it was found that expression of c-Myc and VEGF was significantly downregulated in siRNA-transfected cells and the downregulation of c-Myc and VEGF inhibited cell growth and induced apoptosis and metastasis of Volo cells. c-Myc and VEGF downregulation also increased cell population in the G0–G1 phase. In conclusion, the specific siRNA efficiently silenced the expression of c-Myc and VEGF, further suppressed the cell proliferation, triggered cell apoptosis, and inhibited cell invasiveness of colorectal cancer Volo cells. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:499-505, 2012;Viewthis article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21455

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