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Inhibition of Human MDR1 and BCRP Transporter ATPase Activity by Organochlorine and Pyrethroid Insecticides

Authors

  • Kristin M. Bircsak,

    1. Department of Pharmacology and Toxicology, Rutgers University Ernest Mario School of Pharmacy, Piscataway, USA
    2. Joint Graduate Program in Toxicology, Rutgers University/University of Medicine and Dentistry of New Jersey, Piscataway, NJ, USA
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  • Jason R. Richardson,

    Corresponding author
    1. Environmental and Occupational Health Sciences Institute, a Joint Institute of Robert Wood Johnson Medical School and Rutgers University, Piscataway, NJ, USA
    2. Department of Environmental and Occupational Medicine, University of Medicine and Dentistry of New Jersey, Piscataway, NJ, USA
    • Department of Pharmacology and Toxicology, Rutgers University Ernest Mario School of Pharmacy, Piscataway, USA
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  • Lauren M. Aleksunes

    Corresponding author
    1. Environmental and Occupational Health Sciences Institute, a Joint Institute of Robert Wood Johnson Medical School and Rutgers University, Piscataway, NJ, USA
    • Department of Pharmacology and Toxicology, Rutgers University Ernest Mario School of Pharmacy, Piscataway, USA
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  • Jason R. Richardson and Lauren M. Aleksunes contributed equally as corresponding authors.

  • Contract Grant Sponsor: National Institutes of Health Institute of Diabetes and Digestive and Kidney Diseases.

    Contract Grant number: DK0807741.

    Contract Grant Sponsor: National Institutes of Health Environmental Health Sciences.

    Contract Grant numbers: ES020522, ES015991, ES007148.

    Contract Grant Sponsor: NIEHS Center for Environmental Exposures and Disease.

    Contract Grant number: P30ES005022.

Correspondence to: Jason R. Richardson and Lauren Aleksunes.

ABSTRACT

Despite the growing evidence suggesting that pesticides contribute to chronic diseases, there is a limited understanding of how these chemicals are removed from cells and whether pesticides can alter the disposition of drugs. The present study examined the effects of two classes of insecticides (organochlorine and pyrethroid) on the ATPase activity of the human multidrug resistance protein 1 (MDR1) and breast cancer resistance protein (BCRP) efflux transporters. Using plasma membranes from cells overexpressing MDR1 and BCRP, it was demonstrated that the organochlorine pesticide dichlorodiphenyltrichloroethane (DDT) (o,p'-DDT and p,p'-DDT isomers) as well as its metabolite (p,p'-dichlorodiphenyldichloroethane), inhibit both MDR1 and BCRP ATPase activity. In addition, p,p'-dichlorodiphenyldichloroethylene, and two pyrethroid pesticides inhibited BCRP ATPase activity between 4 and 7 μM. Additional research is necessary to further characterize the functional inhibition of MDR1 and BCRP activity and determine whether pesticides alter the transporter-mediated disposition of other chemicals. © 2012 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:157-164, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21458

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