Protein Compositional Analysis of the Eggs of Black Widow Spider (Latrodectus tredecimguttatus): Implications for the Understanding of Egg Toxicity

Authors


  • Contract Grant Sponsor: National Natural Science Foundation of China.

    Contract Grant Number: 31070700.

    Contract Grant Sponsor: National Basic Research Program or “973 Program” of China.

    Contract Grant Number: 2010CB529800.

    Contract Grant Sponsor: Hunan Provincial Natural Science Foundation of China.

    Contract Grant Number: 11JJ2019.

    Contract Grant Sponsor: Specialized Research Foundation for the doctorial Program of Higher Education of China.

    Contract Grant Number: 20094306110003.

Correspondence to: X. Wang; S. Liang.

ABSTRACT

Previous work found that high-molecular-weight fractions in the egg extract of Latrodectus tredecimguttatus exhibited strong toxicities. For investigating the possible relationship of proteins in the eggs with the toxic effect, the protein composition of the eggs was analyzed using proteomic strategies and compared with that of the spider's venom. SDS-PAGE showed that the proteins of eggs were primarily distributed in the molecular weight range of higher than 55 kDa as well as around 34 kDa, having high abundance proteins with molecular weights of about 60 kDa and 130 kDa. A total of 157 proteins were identified from the egg extract, which were involved in important cellular functions and processes including catalysis, transport, and metabolism regulation. Comparison indicated that the protein composition of eggs is more complex than that of venom, and there are few similarities between the protein composition of the two materials, demonstrating that the eggs have their own distinct toxic mechanism. © 2012Wiley Periodicals, Inc. J BiochemMol Toxicol 26:510-515, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21460

Ancillary