Preventive Effects of (–) Epicatechin on Altered Adenosine Triphosphatases and Minerals in Isoproterenol-Induced Myocardial Infarcted Rats


  • Contract Grant Sponsor: Department of Science and Technology, New Delhi, India.

Correspondence to: P. Stanely Mainzen Prince.


The present study was aimed to evaluate the preventive effects of (–) epicatechin on alterations in the activities/levels of adenosine triphosphatases and minerals in isoproterenol-induced myocardial infarcted rats. Male albino Wistar rats were pretreated with (–) epicatechin (20 mg/kg body weight) daily for a period of 21 days. After the pretreatment period, rats were induced myocardial infarction by isoproterenol (100 mg/kg body weight) on 22nd and 23rd day. The activity of sodium/potassium-dependent adenosine triphosphatase was decreased, and the activities of calcium- and magnesium-dependent adenosine triphosphatases were increased in the heart of isoproterenol-induced myocardial infarcted rats. In addition, the concentrations of potassium were decreased and the concentrations of sodium and calcium were increased in the heart of isoproterenol-induced rats. Elevated plasma lipid peroxidation was noted in isoproterenol-induced rats. Prior treatment with (–) epicatechin significantly prevented the alterations in the activities and concentrations of adenosine triphosphatases, minerals, and plasma lipid peroxidation. The in vitro study confirmed the reducing property of (–) epicatechin. The observed effects in this study are attributed to the membrane-stabilizing and antioxidant properties of (–) epicatechin. The findings of this study will be beneficial to prevent the occurrence of myocardial infarction. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:516-521, 2012; View this article online at DOI 10.1002/jbt.21461