The authors declare that there are no conflicts of interest.
Variation in the Composition and In Vitro Proinflammatory Effect of Urban Particulate Matter from Different Sites
Article first published online: 17 JAN 2013
© 2013 Wiley Periodicals, Inc.
Journal of Biochemical and Molecular Toxicology
Special Issue: Special Issue 1: National Institute of Environmental Health Sciences Outstanding New Environmental Scientist Program
Volume 27, Issue 1, pages 87–97, January 2013
How to Cite
Manzano-León, N., Quintana, R., Sánchez, B., Serrano, J., Vega, E., Vázquez-López, I., Rojas-Bracho, L., López-Villegas, T., O'Neill, M. S., Vadillo-Ortega, F., De Vizcaya-Ruiz, A., Rosas, I. and Osornio-Vargas, Á. R. (2013), Variation in the Composition and In Vitro Proinflammatory Effect of Urban Particulate Matter from Different Sites. J. Biochem. Mol. Toxicol., 27: 87–97. doi: 10.1002/jbt.21471
Contract Grant Sponsor: Fogarty International Research Collaboration Award (FIRCA).
Contract Grant Sponsor: National Institute for Environmental Health Sciences (NIEHS)
Contract Grant Numbers: R01 ES016932 and R01 ES017022-01.
Contract Grant Sponsor: Instituto Nacional de Ecología (INE-Mexico).
- Issue published online: 17 JAN 2013
- Article first published online: 17 JAN 2013
- Manuscript Accepted: 8 DEC 2012
- Manuscript Revised: 6 DEC 2012
- Manuscript Received: 31 OCT 2012
- Fogarty International Research Collaboration Award (FIRCA)
- National Institute for Environmental Health Sciences (NIEHS). Grant Numbers: R01 ES016932, R01 ES017022-01
- Instituto Nacional de Ecología (INE-Mexico)
- Particulate Matter (PM);
- Chemical Composition;
- Spatial Variation;
Spatial variation in particulate matter–related health and toxicological outcomes is partly due to its composition. We studied spatial variability in particle composition and induced cellular responses in Mexico City to complement an ongoing epidemiologic study. We measured elements, endotoxins, and polycyclic aromatic hydrocarbons in two particle size fractions collected in five sites. We compared the in vitro proinflammatory response of J774A.1 and THP-1 cells after exposure to particles, measuring subsequent TNFα and IL-6 secretion. Particle composition varied by site and size. Particle constituents were subjected to principal component analysis, identifying three components: C1 (Si, Sr, Mg, Ca, Al, Fe, Mn, endotoxin), C2 (polycyclic aromatic hydrocarbons), and C3 (Zn, S, Sb, Ni, Cu, Pb). Induced TNFα levels were higher and more heterogeneous than IL-6 levels. Cytokines produced by both cell lines only correlated with C1, suggesting that constituents associated with soil induced the inflammatory response and explain observed spatial differences. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:87-97, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21471