Prooxidative Effects of Ambient Pollutant Chemicals Are Inhibited by HDL
Article first published online: 18 FEB 2013
© 2013 Wiley Periodicals, Inc.
Journal of Biochemical and Molecular Toxicology
Special Issue: Special Issue 2: National Institute of Environmental Health Sciences Outstanding New Environmental Scientist Program
Volume 27, Issue 2, pages 172–183, February 2013
How to Cite
Yin, F., Ramanathan, G., Zhang, M. and Araujo, J. A. (2013), Prooxidative Effects of Ambient Pollutant Chemicals Are Inhibited by HDL. J. Biochem. Mol. Toxicol., 27: 172–183. doi: 10.1002/jbt.21475
- Issue published online: 18 FEB 2013
- Article first published online: 18 FEB 2013
- Manuscript Accepted: 11 JAN 2013
- Manuscript Revised: 26 DEC 2012
- Manuscript Received: 4 OCT 2012
- Contract Grant Sponsor: National Institute of Environmental Health Sciences.
- Contract Grant Sponsor: National Institutes of Health (ONES RO1 Award) (to JAA).. Grant Number: Contract Grant Number: ES016959.
- diesel exhaust particles;
- oxidative stress;
Exposure to air pollution leads to adverse pulmonary and systemic vascular effects. High levels of plasma high-density lipoproteins (HDL) cholesterol reduce cardiovascular risk. We explored whether HDL could protect against the prooxidative effects of an organic extract of diesel exhaust particles (DEP) in vascular cells. We used a cell-free fluorescent assay to evaluate DEP oxidation by air, estimated by the degree of dichlorofluorescein (DCF) fluorescence and tested the ability of HDL to inhibit it. We also evaluated DEP prooxidative effects in bovine aortic endothelial cells and RAW264.7 macrophages by DCF fluorescence. DEP oxidation and prooxidative cellular effects occurred in concentration- and time-dependent manners. Normal HDL inhibited DEP oxidation and prooxidative cellular effects, whereas dysfunctional HDL failed to inhibit DEP oxidation and instead, it promoted further oxidation. In conclusion, DEP prooxidative effects in endothelial cells and macrophages are inhibited by normal HDL. Therefore, HDL may protect against air pollution mediated adverse vascular effects. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:172-183, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21475