Jinwen Liu, Weixuan Li, Xiaoyu Piao, and Junwen Zhang contributed equally to this work.
Icariside II Reduces Testosterone Production by Inducing Necrosis in Rat Leydig Cells
Article first published online: 22 MAR 2013
© 2013 Wiley Periodicals, Inc.
Journal of Biochemical and Molecular Toxicology
Volume 27, Issue 4, pages 243–250, April 2013
How to Cite
Liu, J., Li, W., Piao, X., Zhang, J., Zhang, D., Wei, N., Hu, D. and Liu, S. (2013), Icariside II Reduces Testosterone Production by Inducing Necrosis in Rat Leydig Cells. J. Biochem. Mol. Toxicol., 27: 243–250. doi: 10.1002/jbt.21481
Contract grant sponsor: Fundamental Research Funds for the Central Universities.
Contract grant number: YX2013-22.
- Issue published online: 3 APR 2013
- Article first published online: 22 MAR 2013
- Manuscript Accepted: 22 FEB 2013
- Manuscript Revised: 29 JAN 2013
- Manuscript Received: 19 NOV 2012
- Fundamental Research Funds for the Central Universities. Grant Number: YX2013-22
- Icariside II;
- Leydig Cell;
The present study demonstrates that Icariside II (10, 20, and 40 µM) reduced Leydig cell testosterone production and cell viability in a concentration- and time-dependent manner. Hoechst 33342/propidium iodide staining indicated that no morphological changes in Leydig cell nuclear chromatin occurred, caspase-3 expression also showed no significant change, but cell death was caused by the 10-µM Icariside II treatment. Furthermore, a significant reduction in NAD+ levels was observed following Icariside II exposure (10, 20, and 40 µM). Cell death was avoided when Icariside II treated cells were incubated with extracellular NAD+ (5 and 10 mM). Moreover, the addition of NAD+ (5 and 10 mM) could restore ATP production and prevent cell death. The results suggest that Icariside II can reduce testosterone production by inducing necrosis, but not apoptosis, in rat Leydig cells. This mechanism may also account for the Icariside II induced depletion of NAD+ and ATP levels. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:243-250, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21481