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Study on the Interaction Mechanism of 2-Aminoanthraquinone with Calf Thymus DNA

Authors

  • Hongxu Yang,

    1. School of Environmental Science and Engineering, Shandong University, China –America CRC for Environment & Health, Shandong Province, Jinan, People's.Republic of China
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  • Wei Song,

    1. School of Environmental Science and Engineering, Shandong University, China –America CRC for Environment & Health, Shandong Province, Jinan, People's.Republic of China
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  • Mingyang Jing,

    1. School of Environmental Science and Engineering, Shandong University, China –America CRC for Environment & Health, Shandong Province, Jinan, People's.Republic of China
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  • Rutao Liu

    Corresponding author
    • School of Environmental Science and Engineering, Shandong University, China –America CRC for Environment & Health, Shandong Province, Jinan, People's.Republic of China
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  • Contract grant Sponsor: National Science Foundation of China.

  • Contract grant number: 21277081.

  • Contract grant Sponsor: Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China.

  • Contract grant number: 708058.

  • Contract grant Sponsor: Independent innovation program of Jinan.

  • Contract grant number: 201202083.

  • Contract grant Sponsor: Independent innovation foundation of Shandong University natural science projects.

  • Contract grant number: 2012DX002.

Correspondence to: Rutao Liu.

ABSTRACT

By utilizing multispectrosopic techniques, the toxic interaction of 2-aminoanthraquinone (2-AAQ) with calf thymus deoxyribonucleic acid (ctDNA) was investigated in vitro under simulated physiological conditions. The experimental results proved that 2-AAQ has a toxic interaction with ctDNA. The binding capacity of DNA with 2-AAQ is diminishing as the pH value of system increasing in the optimization of experimental condition. Moreover we selected pH 7.4, which is nearly physiological condition to enhance the practical significance. According to the Stern–Volmer equation, the quenching was the static quenching process. And the quenching constant math formula can be derived from the fluorescence quenching spectrogram. Ultraviolet absorption spectra and the change in the fluorescence intensity at different ionic strengths further indicated that there was electrostatic binding between 2-AAQ and ctDNA. The circular dichroism experiment showed that the DNA conformation varied from B to A conformation. The basic group enhanced after 2-AAQ embedding. The double helix is more compact, and the DNA conformation changes. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:272-278, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21487

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