Jianjun Li and Yizhong Yan made equal contributions to this work.
Purification and Partial Characterization of a Novel Neurotoxic Protein from Eggs of Black Widow Spiders (Latrodectus tredecimguttatus)
Article first published online: 13 MAY 2013
© 2013 Wiley Periodicals, Inc.
Journal of Biochemical and Molecular Toxicology
Volume 27, Issue 7, pages 337–342, July 2013
How to Cite
Li, J., Yan, Y., Wang, J., Guo, T., Hu, W., Duan, Z., Wang, X. and Liang, S. (2013), Purification and Partial Characterization of a Novel Neurotoxic Protein from Eggs of Black Widow Spiders (Latrodectus tredecimguttatus). J. Biochem. Mol. Toxicol., 27: 337–342. doi: 10.1002/jbt.21493
Contract grant sponsor: National Natural Science Foundation of China.
Contract grant number: 31070700.
Contract grant sponsor: National Basic Research Program or “973 Program” of China.
Contract grant number: 2010CB529800.
Contract grant sponsor: Hunan Provincial Natural Science Foundation of China.
Contract grant number: 11JJ2019.
- Issue published online: 2 JUL 2013
- Article first published online: 13 MAY 2013
- Manuscript Accepted: 20 APR 2013
- Manuscript Revised: 10 APR 2013
- Manuscript Received: 18 FEB 2013
- National Natural Science Foundation of China. Grant Number: 31070700
- National Basic Research Program or “973 Program” of China. Grant Number: 2010CB529800
- Hunan Provincial Natural Science Foundation of China. Grant Number: 11JJ2019
- Neurotoxic Protein;
- Black Widow Spider;
Up to now, there have been a few reports on the toxic components purified from black widow spider (Latrodectus tredecimguttatus) eggs. In the present study, a novel neurotoxic protein was purified from the eggs by gel filtration combined with ion-exchange chromatography. Its molecular weight was 23.752 kDa determined by electrospray mass spectrometry. The protein could block the neuromuscular transmission in mouse-isolated phrenic nerve-hemidiaphragm preparations completely in a reversible manner and activate tetrodotoxin-sensitive sodium current in rat dorsal root ganglion cells. The N-terminal sequence of the protein was identified by the Edman degradation to be N-S-I-A-D-D-R-Y-R-W-P-G-Y-P-G-A-G-L-I-P-Y-I-I-D-S—. When the sequence was used to search against protein database with a sequence query in Mascot engine there was no matched sequence or protein whereas the Basic Local Alignment Search Tool (BLAST) analysis indicated that no significant similarity was found. These results demonstrated that the protein (named Latroeggtoxin-I) is a novel neurotoxic protein purified from the eggs of black widow spiders. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:337-342, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21493