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Purification and Partial Characterization of a Novel Neurotoxic Protein from Eggs of Black Widow Spiders (Latrodectus tredecimguttatus)

Authors

  • Jianjun Li,

    1. Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China
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  • Yizhong Yan,

    1. Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China
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  • Jirong Wang,

    1. Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China
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  • Tianyao Guo,

    1. Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China
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  • Weijun Hu,

    1. Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China
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  • Zhigui Duan,

    1. Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China
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  • Xianchun Wang,

    Corresponding author
    • Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China
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  • Songping Liang

    Corresponding author
    • Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China
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  • Jianjun Li and Yizhong Yan made equal contributions to this work.

  • Contract grant sponsor: National Natural Science Foundation of China.

  • Contract grant number: 31070700.

  • Contract grant sponsor: National Basic Research Program or “973 Program” of China.

  • Contract grant number: 2010CB529800.

  • Contract grant sponsor: Hunan Provincial Natural Science Foundation of China.

  • Contract grant number: 11JJ2019.

Correspondence to: X. Wang and S. Liang.

ABSTRACT

Up to now, there have been a few reports on the toxic components purified from black widow spider (Latrodectus tredecimguttatus) eggs. In the present study, a novel neurotoxic protein was purified from the eggs by gel filtration combined with ion-exchange chromatography. Its molecular weight was 23.752 kDa determined by electrospray mass spectrometry. The protein could block the neuromuscular transmission in mouse-isolated phrenic nerve-hemidiaphragm preparations completely in a reversible manner and activate tetrodotoxin-sensitive sodium current in rat dorsal root ganglion cells. The N-terminal sequence of the protein was identified by the Edman degradation to be N-S-I-A-D-D-R-Y-R-W-P-G-Y-P-G-A-G-L-I-P-Y-I-I-D-S—. When the sequence was used to search against protein database with a sequence query in Mascot engine there was no matched sequence or protein whereas the Basic Local Alignment Search Tool (BLAST) analysis indicated that no significant similarity was found. These results demonstrated that the protein (named Latroeggtoxin-I) is a novel neurotoxic protein purified from the eggs of black widow spiders. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:337-342, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21493

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